G-CSF, Human, Recombinant, E Coli | MyBioSource MBS203079 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

G-CSF, Human, Recombinant, E Coli

catalog :

MBS203079

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203079

products type :

Recombinant Protein

products full name :

G-CSF, Human, Recombinant, E Coli

products short name :

G-CSF

products name syn :

CSF3; GCSF; G-CSF; G-CSF; Pluripoietin; Filgrastim; Lenograstim; Granulocyte colony-stimulating factor; Granulocyte-colony stimulating factor Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; Csfg; GCSA; Granulocyte colony stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2

other names :

granulocyte colony-stimulating factor isoform b; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin; INN: Filgrastim; INN: Lenograstim

products gene name :

G-CSF

other gene names :

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

uniprot entry name :

CSF3_HUMAN

sequence length :

204

sequence :

MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

purity :

> 95% by SDS - PAGE

form :

Liquid. In phosphate-buffered Saline(PBS), pH7.4, 10% glycerol

concentration :

0.5mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: The proliferative activity of rhG-CSF is tested in culture using mouse bone marrow cells. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Hormones & Cytokines

products description :

Human granulocyte colony-stimulating factor(hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

products references :

Nomura H, et al.(1986) EMBO J, 5(5): 871-6. Nagata, S., et al.(1986) Nature, 319 : 415-418. Demetri, G. D., et al.(1991) Blood, 78: 2791-2808 . Hill, C. P., et al (1993) Proc. Natl. Acad. Sci. uSA, 90:5167-5171.

ncbi gi num :

27437049

ncbi acc num :

NP_757373

ncbi gb acc num :

NM_172219.2

ncbi mol weight :

18.8kDa (175aa), confirmed by MALDI-TOF.

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Malaria Pathway (152665); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

uniprot summary :

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space. Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding. Biological Process: granulocyte differentiation; positive regulation of protein binding; positive regulation of myeloid cell differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage