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Growth Hormone, Human Recombinant, E Coli

MBS203077

0.1 mg

255 USD

MBS203077

Recombinant Protein

Growth Hormone, Human Recombinant, E Coli

Growth Hormone

GH1; Growth Hormone; GH; GH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin; GHN; hGH-N

somatotropin isoform 1; Somatotropin; somatotropin; pituitary growth hormone; growth hormone 1; Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone

GH1; GH1; GH; GHN; GH-N; hGH-N; IGHD1B; GH; GH-N

SOMA_HUMAN

217

MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF

> 95% by SDS - PAGE

Liquid. In. 88mM mannitol, 5mM phosphate, pH7.4

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Measured in a cell proliferation assay using Nb2 Rat lymphoma cells. The ED50 for this effect is 1. Cell line: Nb2-11 (Rat lymphoma cell) . 2. Maintenance Condition: 10% FBS, 10% HS, 50uM 2-ME, RPMI 1640 . 3. Assay Medium: 10% HS, 50uM 2-ME, RPMI 1640 (without FBS) . 4. Cell Density: 1 x 104 cells/well (96 well plate, final volume 100ul) . 5. Starvation : 24hr, Assay medium . 6. Incubation Time: 48hr (after sample treatment) . 7. Concentration Range: 0.002 ng/ml - 10 ng/ml . 8. Detection Method : CCK-8 assay .

Hormones & Cytokines

Human growth hormone(hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Its main function is the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant human growth hormone(rhGH) was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Wickens., et al.(1978) J. Biol Chem. 253(7):2483-2495. Goeddel. et al.(1979) Proc. Natl. Acad. Sci. 76:106-110. Chang, et al. (1978) Nature 275:617-624 . ullrich. et al. (1977) Science 196:1313-1319.

13027812

NP_000506

NM_000515.4

22.2 kDa (192 aa), confirmed by MALDI-TOF.

Adipogenesis Pathway 198832!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Endochondral Ossification Pathway 198812!!Growth Hormone Receptor Signaling Pathway 477128!!Immune System Pathway 106386!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!Metabolism Of Proteins Pathway 106230

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

0.1 mg

255 USD