Recombinant Platelet Derived Growth Factor Receptor Beta (PDGFRb) | MyBioSource MBS2030725 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Platelet Derived Growth Factor Receptor Beta (PDGFRb)

catalog :

MBS2030725

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

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product information

catalog number :

MBS2030725

products type :

Recombinant Protein

products full name :

Recombinant Platelet Derived Growth Factor Receptor Beta (PDGFRb)

products short name :

[Platelet Derived Growth Factor Receptor Beta]

other names :

[platelet-derived growth factor receptor beta; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta; platelet derived growth factor receptor, beta polypeptide; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD_antigen: CD140b]

products gene name :

[PDGFRb]

other gene names :

[Pdgfrb; Pdgfrb; PDGFR-1; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1]

uniprot entry name :

PGFRB_RAT

host :

Host: E Coli. Source: Prokaryotic expression

sequence length :

1097

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VVFE AYPMPSVLWL KDNRTLGDSG AGELVLSTRN MSETRYVSEL ILVRVKVSEA GYYTMRAFHE DDEVQLSFKL QVNVPVRVLE LSESHPANGE QTIRCRGRGM PQPNVTWSTC RDLKRCPRKL SPTPLGNSSK EESQLETNVT FWEEDQEYEV VSTLRLRHVD QPLSVRCMLQ NSMGGDSQEV TVVPHSLPFK VVVISAILAL VVLTVISLII LIMLWQKKPR YEIRWKVIES VSSDGHEYIY VDP

purity :

>95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

concentration :

6.5

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

image3 heading :

Testing Data

other info1 :

Predicted isoelectric point: 6.5. Predicted Molecular Mass: 32.0kDa . Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Tags: N-terminal His-Tag. Tissue Specificity: Fibroblast, Intestine, Myoblast. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen.

other info2 :

Expression System: Prokaryotic expression. Tag: two N-terminal Tags, His-tag and T7-tag. Organism Species: Mus musculus (Mouse). Fragment: Val337~Pro583. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

29789275

ncbi acc num :

NP_113713.1

ncbi gb acc num :

NM_031525.1

ncbi mol weight :

122,828 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1262776); Adaptive Immune System Pathway (1263039); Axon Guidance Pathway (1262465); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Central Carbon Metabolism In Cancer Pathway (1060543); Central Carbon Metabolism In Cancer Pathway (1084231); Choline Metabolism In Cancer Pathway (1060542); Choline Metabolism In Cancer Pathway (1084232); Cytokine Signaling In Immune System Pathway (1263157)

ncbi summary :

binds platelet derived growth factor BB homodimer [RGD, Feb 2006]

uniprot summary :

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Oncoprotein; TK group; PDGFR family

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

265

size3 :

0.1 mg

price3 :

410

size4 :

0.2 mg

price4 :

500

size5 :

0.5 mg

price5 :

975

size6 :

1 mg

price6 :

1440

more info or order :

MyBioSource product webpage