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protein

ubiquitin Human, Recombinant, expressed in E Coli

MBS203070

0.1 mg

255 USD

MBS203070

Recombinant Protein

ubiquitin Human, Recombinant, expressed in E Coli

ubiquitin

RPS27A; uBA80; uBCEP1; uBA52; uBCEP2; uBB; uBC; ubiquitin; FLJ25987; MGC8385; Polyubiquitin B; ubiquitin B

ubiquitin; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a; epididymis luminal protein 112; ubiquitin C; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ubiquitin-CEP80; ribosomal protein S27a; Ubiquitin carboxyl extension protein 80Cleaved into the following 2 chains:Ubiquitin; 40S ribosomal protein S27a

RPS27A; RPS27A; UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80; UBA80; UBCEP1

RS27A_HUMAN

156

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

> 95% by SDS - PAGE

Liquid. In 50 mM HEPES (pH7.5) 150mM NaCl, 1mM DTT, 10%glycerol.

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli

ubiquitination

ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity. ubiquitin(76amino acid) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Lund PK., et al. (1985) J Biol. Chem. 260(12), 7609-7613. Pancre V., et al. (1991) Eur J Immunol. 21(11):2735-41.

37571

CAA44911

P62979

8.6 kDa (76 aa), confirmed by MALDI-TOF.

AMER1 Mutants Destabilize The Destruction Complex Pathway 1127621!!APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway 1127616!!APC Truncation Mutants Have Impaired AXIN Binding Pathway 1127617!!APC-Cdc20 Mediated Degradation Of Nek2A Pathway 1127551!!APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway 105832!!APC/C:Cdc20 Mediated Degradation Of Securin Pathway 105833!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 105835!!APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway 1127549

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]

0.1 mg

255 USD