catalog number :
MBS2030668
products type :
Recombinant Protein
products full name :
Recombinant P-Selectin Glycoprotein Ligand 1 (PSGL1)
products short name :
[P-Selectin Glycoprotein]
products gene name :
[PSGL1]
concentration :
Original Concentration: 200 Ug/mL
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
SDS-Page
other info1 :
Organism Species: Mus musculus (Mouse). Source: Prokaryotic expression. Residues: Ser94~Ser345. Tags: N-terminal His and GST Tag. Subcellular Location: Membrane, Chromosome. Traits: Freeze-dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
other info2 :
Predicted isoelectric point: 5.3. Predicted Molecular Mass: 56.2kDa. Accurate Molecular Mass: 70/65kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved.
to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not
vortex.