RXR-alpha(Retinoid x receptor;111-228aa, Human) Recombinant, expressed in E Coli | MyBioSource MBS203060 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

RXR-alpha(Retinoid x receptor;111-228aa, Human) Recombinant, expressed in E Coli

catalog :

MBS203060

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203060

products type :

Recombinant Protein

products full name :

RXR-alpha(Retinoid x receptor;111-228aa, Human) Recombinant, expressed in E Coli

products short name :

RXR-alpha

products name syn :

RXRA; NR2B1; RXR-alpha; Retinoid x receptor; Retinoid X receptor alpha; Nuclear receptor subfamily 2 group B member 1; Retinoic acid receptor RXR-alpha; FLJ16020; FLJ16733; MGC102720; Retinoic acid receptor RXR alpha; RXR alpha1; RXRalpha1; RXRA1

other names :

retinoic acid receptor RXR-alpha isoform a; Retinoic acid receptor RXR-alpha; retinoic acid receptor RXR-alpha; nuclear receptor subfamily 2 group B member 1; retinoid X nuclear receptor alpha; retinoid X receptor, alpha; Nuclear receptor subfamily 2 group B member 1; Retinoid X receptor alpha

products gene name :

RXR-alpha

other gene names :

RXRA; RXRA; NR2B1; NR2B1

uniprot entry name :

RXRA_HUMAN

sequence length :

462

sequence :

MLGLNGVLKV PAHPSGNMAS FTKHICAICG DRSSGKHYGV YSCEGCKGFF KRTVRKDLTY TCRDNKDCLI DKRQRNRCQY CRYQKCLAMG MKREAVQEER QRGKDRNENE VESTSSANE

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl pH7.5, 0.1M NaCl, 5mM beta-Mercaptoethanol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli

products categories :

Cell Cycle

products description :

The retinoid X receptor(RXR) is a pleiotropic nuclear receptor transcription factor that interacts with a variety of nuclear receptor dimeric partner. RXR binds cognate response elements as a homodimer in the presence of its ligand, 9-cis retinoic acid, or as a heterodimer with other members of the nuclear hormone receptor superfamily including retinoic acid receptors(RAR), thyroid hormone receptors(TR), vitamin D receptors and peroxisome proliferators-activated receptors(PPAR). The RXR family includes three difference isoforms ; RXR alpha, beta, gamma. Human RXR alpha gene is localized on 9q34.9 and encodes two major isoforms(RXR alpha1, RXR alpha2). The DNA binding domain of RXR(111-228aa) was over expressed in E.coli and purified by using conventional column chromatography techniques.

products references :

Si J., et al. (2002) Blood, 100(13);4401-9. Zeng M., et al. (2002) J. Biol. Chem. 277(47):45611-8.

ncbi gi num :

4506755

ncbi acc num :

NP_002948

ncbi gb acc num :

NM_002957.5

ncbi mol weight :

13.6 kDa (119 aa), confirmed by MALDI-TOF.

ncbi pathways :

Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Bile Acid And Bile Salt Metabolism Pathway (106144); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Biological Oxidations Pathway (105698); Circadian Clock Pathway (187173)

ncbi summary :

Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

RXRA: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5 -AGGTCA-3 sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5 -AGGTCA-3 sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. Homodimer. Heterodimer with RARA; required for ligand- dependent retinoic acid receptor transcriptional activity. Heterodimer with PPARA (via the leucine-like zipper in the LBD); the interaction is required for PPARA transcriptional activity. Also heterodimerizes with PPARG. Interacts with NCOA3 and NCOA6 coactivators. Interacts with FAM120B. Interacts with PELP1, SENP6, SFPQ, DNTTIP2 and RNF8. Interacts (via the DNA binding domain) with HCV core protein; the interaction enhances the transcriptional activities of the RXRA/RARA and the RXRA/PPARA heterodimers. Interacts with PRMT2. Interacts with ASXL1 and NCOA1. Highly expressed in liver, also found in lung, kidney and heart. Belongs to the nuclear hormone receptor family. NR2 subfamily. Protein type: Motility/polarity/chemotaxis; DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex. Molecular Function: ligand-dependent nuclear receptor activity; zinc ion binding; chromatin DNA binding; transcription coactivator activity; protein binding; enzyme binding; DNA binding; retinoid-X receptor activity; vitamin D receptor binding; protein heterodimerization activity; sequence-specific DNA binding; steroid hormone receptor activity; retinoic acid receptor activity; transcription factor activity. Biological Process: retinoic acid receptor signaling pathway; cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; camera-type eye development; vitamin metabolic process; response to retinoic acid; in utero embryonic development; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; positive regulation of translational initiation by iron; protein homotetramerization; virus-host interaction; ventricular cardiac muscle morphogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; transmembrane transport; embryo implantation; maternal placenta development

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage