Pin 1(Peptidyl-prolyl cis/trans isomerase) Human, Recombinant, E Coli | MyBioSource MBS203059 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Pin 1(Peptidyl-prolyl cis/trans isomerase) Human, Recombinant, E Coli

catalog :

MBS203059

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203059

products type :

Recombinant Protein

products full name :

Pin 1(Peptidyl-prolyl cis/trans isomerase) Human, Recombinant, E Coli

products short name :

Pin 1

products name syn :

PIN1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; EC 5.2.1.8; Rotamase Pin1; PPIase Pin1; DOD; uBL5; PIN1; PPIase; EC 5.2.1.8; Rotamase Pin1; PPIase Pin1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

other names :

peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PPIase Pin1; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; rotamase Pin1; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1

products gene name :

Pin 1

other gene names :

PIN1; PIN1; DOD; UBL5; PPIase Pin1

uniprot entry name :

PIN1_HUMAN

sequence length :

163

sequence :

MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

purity :

95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer(pH7.5) 0.1M NaCl, 5mM DTT, 20%Glycerol.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant PIN 1 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

products categories :

Cell Cycle

products description :

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

products references :

Wulf GM., et al. (2002) J Biol. Chem. 277(50):47976-47979. Hamdane M., et al. (2002) J Mol Neurosci. 19(3); 275-287. Zheng H, et al. (2002) Nature. 419(6909) 853-857.

ncbi gi num :

5453898

ncbi acc num :

NP_006212

ncbi gb acc num :

NM_006221.3

ncbi mol weight :

18.2 kDa (163 aa), confirmed by MALDI-TOF.

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway 530760!!C-MYC Pathway 169344!!Cytokine Signaling In Immune System Pathway 366171!!ISG15 Antiviral Mechanism Pathway 530761!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!Interferon Signaling Pathway 187103!!Negative Regulators Of RIG-I/MDA5 Signaling Pathway 187102!!RIG-I-like Receptor Signaling Pathway 117292!!RIG-I-like Receptor Signaling Pathway 116128

ncbi summary :

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

size :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage