protein

Recombinant Paraoxonase 3 (PON3)

MBS2030563

0.01 mg

140 USD

Recombinant Protein

Recombinant Paraoxonase 3 (PON3)

[Paraoxonase 3]

[paraoxonase 3; Serum paraoxonase/lactonase 3; serum paraoxonase/lactonase 3; paraoxonase 3]

[PON3]

[PON3; PON3]

PON3_HUMAN

E.coli

> 97%

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 24 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Gly2~Leu354. Tags: N-terminal His Tag. Subcellular Location: Secreted. Traits: Freeze-dried powder. Buffer: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300

Predicted isoelectric point: 5.2. Predicted Molecular Mass: 43.2kDa. Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

51094887

EAL24132.1

Metabolic Pathways (132956); Phase I, Non P450 Pathway (198854)

This gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein (LDL), a function that is believed to slow the initiation and progression of atherosclerosis. Alternatively spliced variants which encode different protein isoforms have been described; however, only one has been fully characterized. [provided by RefSeq, Jul 2008]

PON3: Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents. Belongs to the paraoxonase family. Protein type: EC 3.1.1.81; EC 3.1.1.2; Secreted, signal peptide; Hydrolase; EC 3.1.8.1; Secreted. Chromosomal Location of Human Ortholog: 7q21.3. Cellular Component: extracellular space; intracellular membrane-bound organelle. Molecular Function: protein homodimerization activity; 3,4-dihydrocoumarin hydrolase activity; arylesterase activity; dihydrocoumarin hydrolase activity; metal ion binding; aryldialkylphosphatase activity. Biological Process: response to external stimulus; dephosphorylation; coumarin catabolic process; carboxylic acid catabolic process; aromatic compound catabolic process; phenylacetate catabolic process

0.01 mg

140 USD

0.05 mg

260

0.1 mg

400

0.2 mg

495

0.5 mg

955

1 mg

1415