protein

14-3-3 epsilon, 1-255aa, Human, Recombinant, E Coli

MBS203051

0.1 mg

375 USD

Recombinant Protein

14-3-3 epsilon, 1-255aa, Human, Recombinant, E Coli

14-3-3 epsilon

YWHAE; MDS; MDCR; KCIP-1; FLJ45465; 14-3-3 epsilon; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; epsilon polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; epsilon polypeptide 14 3 3 E; 14 3 3E; KCIP 1; KCIP1; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein1; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.

14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 epsilon; epididymis luminal protein 2; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon

14-3-3 epsilon

YWHAE; YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E; 14-3-3E

1433E_HUMAN

255

MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ

> 95% by SDS - PAGE

In 20mM Tris pH7.5

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli

Signal Transduction

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E.coli and purified by using conventional chromatography techniques.

Oriente F, et al.(2005) J Biol Chem. 280(49):40642-9. Conklin D, et al.(1995) PNAS. 92(17):7892-6.

5803225

NP_006752

NM_006761.4

29 kDa (255 aa), confirmed by MALDI-TOF.

Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Anchoring Of The Basal Body To The Plasma Membrane Pathway 1127503!!Apoptosis Pathway 105648!!Assembly Of The Primary Cilium Pathway 1127502!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

0.1 mg

375 USD