protein

14-3-3 gamma, 1-247aa, Human, Recombinant, E Coli

MBS203050

0.1 mg

310 USD

Recombinant Protein

14-3-3 gamma, 1-247aa, Human, Recombinant, E Coli

14-3-3 gamma

YWHAG; 14-3-3 gamma; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; gamma polypeptide 14 3 3 protein gamma; 14 3 3 protein gamma subtype; 14 3 3gamma; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide; KCIP 1; KCIP1; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide.

14-3-3 protein gamma; 14-3-3 protein gamma; 14-3-3 protein gamma; 14-3-3 gamma; KCIP-1; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma; Protein kinase C inhibitor protein 1; KCIP-1

14-3-3 gamma

YWHAG; YWHAG; PPP1R170; 14-3-3GAMMA; KCIP-1

1433G_HUMAN

247

MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

> 95% by SDS - PAGE

Liquid in Phosphate buffered saline (pH7.4), 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Signal Transduction

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human YWHAG was expressed in E.coli and purified by using conventional chromatography techniques.

Chen XQ, et al. (2005) Glia. 42(4):315-24 . Chen XQ, Yu AC, et al. (2002) Biochem Biophys Res Commun. 296(3):657-63 .

21464101

NP_036611

NM_012479.3

28 kDa (247 aa), confirmed by MALDI-TOF.

Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Anchoring Of The Basal Body To The Plasma Membrane Pathway 1127503!!Apoptosis Pathway 105648!!Assembly Of The Primary Cilium Pathway 1127502!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

0.1 mg

310 USD