protein

Hexokinase 1 Human, His-tag, Recombinant, E Coli

MBS203049

0.1 mg

510 USD

Recombinant Protein

Hexokinase 1 Human, His-tag, Recombinant, E Coli

Hexokinase 1

HK1; Hexokinase 1; EC 2.7.1.1; Hexokinase type I; HK I; Brain form hexokinase; Hexokinase-1; BB404130; dea; Glycolytic enzyme; HEXOKIN; Hexokinase PI; Hexokinase type I; Hexokinase; tumor isozyme; Hexokinase-A; HK1 tb; Hk1-s; HK1-ta; HK1-tc; HKI; HXK1; mHk1-s.

hexokinase-1 isoform HKI; Hexokinase-1; hexokinase-1; HK I; brain form hexokinase; glycolytic enzyme; hexokinase type I; hexokinase 1; Brain form hexokinase; Hexokinase type I; HK I

HK1; HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc; HK I

HXK1_HUMAN

917

MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl pH8.0, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C. Assay: 1. Prepare a 2.57ml reaction mixture into a suitable container: The final concentrations are 39 mM triethanolamine, 216 mM D-glucose, 0.74mM ATP, 7.8 mM MgCl2, 1.1 mM beta-NADP, 2.5 units G6PD. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 5ug of recombinant hexokinase1 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes

Signal Transduction

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Jon E. et al.,(2003) J.Exp Biology. 206 : 2049-2057. Furuta H. et al.,(1996) Genomics. 36(1):206-9.

188497754

NP_000179

NM_000188.2

104.6kDa (937aa)

Amino Sugar And Nucleotide Sugar Metabolism Pathway 82979!!Amino Sugar And Nucleotide Sugar Metabolism Pathway 350!!Butirosin And Neomycin Biosynthesis Pathway 145809!!Butirosin And Neomycin Biosynthesis Pathway 145786!!Carbohydrate Digestion And Absorption Pathway 170720!!Carbohydrate Digestion And Absorption Pathway 170654!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

0.1 mg

510 USD