protein

Hexokinase 2 Human, His-tag, Recombinant, E Coli

MBS203048

0.1 mg

510 USD

Recombinant Protein

Hexokinase 2 Human, His-tag, Recombinant, E Coli

Hexokinase 2

HK2; Hexokinase 2; EC 2.7.1.1; Hexokinase type II; HK II; Muscle form hexokinase; Hexokinase-2; hexokinase 2

hexokinase-2; Hexokinase-2; hexokinase-2; HK II; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase; hexokinase 2; Hexokinase type II; HK II; Muscle form hexokinase

HK2; HK2; HKII; HXK2; HK II

HXK2_HUMAN

917

MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR

> 85% by SDS - PAGE

Liquid. In 20mM Tris-HCl pH8.0, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C. Assay: 1. Prepare a 2.57ml reaction mixture into a suitable container: The final concentrations are 39mM triethanolamine, 216 mM D-glucose, 0.74mM ATP, 7.8 mM MgCl2, 1.1 mM beta-NADP, 2.5 units G6PD. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 5ug of recombinant hexokinase2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes.

Signal Transduction

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Recombinant human Hexokinase2, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Jon E. et al.,(2003) J.Exp Biology. 206 : 2049-2057. Furuta H. et al.,(1996) Genomics. 36(1):206-9.

15553127

NP_000180

NM_000189.4

104.1kDa (937aa)

Amino Sugar And Nucleotide Sugar Metabolism Pathway 82979!!Amino Sugar And Nucleotide Sugar Metabolism Pathway 350!!Butirosin And Neomycin Biosynthesis Pathway 145809!!Butirosin And Neomycin Biosynthesis Pathway 145786!!Carbohydrate Digestion And Absorption Pathway 170720!!Carbohydrate Digestion And Absorption Pathway 170654!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

0.1 mg

510 USD