protein

Hexokinase 4 Human, His-tag, Recombinant, E Coli

MBS203047

0.1 mg

510 USD

Recombinant Protein

Hexokinase 4 Human, His-tag, Recombinant, E Coli

Hexokinase 4

GCK; Hexokinase 4; EC 2.7.1.2; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D; Glucokinase; ATP D hexose 6 phosphotransferase; GK; GLK; Glucokinase; Glucokinase isoform 1; Hexokinase 4; Hexokinase D; Hexokinase D pancreatic isozyme; Hexokinase4; HexokinaseD; HHF 3; HHF3; HK 4; HXKP; Maturity onset diabetes of the young 2; MODY 2; MODY2.

glucokinase isoform 1; Glucokinase; glucokinase; ATP:D-hexose 6-phosphotransferase; HK IV; hexokinase D, pancreatic isozyme; hexokinase type IV; hexokinase-4; hexokinase-D; glucokinase (hexokinase 4); Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D

GCK; GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3; HK IV; HK4

HXK4_HUMAN

465

MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl pH8.0, 10% glycerol.

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called glucokinase. Recombinant human Hexokinase4, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Jon E. et al.,(2003) J.Exp Biology. 206: 2049-2057

4503951

NP_000153

NM_000162.3

54.3kDa (485aa)

Amino Sugar And Nucleotide Sugar Metabolism Pathway 82979!!Amino Sugar And Nucleotide Sugar Metabolism Pathway 350!!Butirosin And Neomycin Biosynthesis Pathway 145809!!Butirosin And Neomycin Biosynthesis Pathway 145786!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231!!Developmental Biology Pathway 477129!!Disease Pathway 530764

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]

0.1 mg

510 USD