catalog number :
MBS203046
products type :
Recombinant Protein
products full name :
Hexokinase 3 Human, His-tag,Recombinant, E Coli
products short name :
Hexokinase 3
products name syn :
HK3; Hexokinase 3; EC 2.7.1.1; Hexokinase type III; HK III; Hexokinase-3; HKIII; Hexokinase3; HK3; HXK3.
other names :
hexokinase-3; Hexokinase-3; hexokinase-3; ATP:D-hexose 6-phosphotransferase; HK III; hexokinase type III; hexokinase 3 (white cell); Hexokinase type III; HK III
other gene names :
HK3; HK3; HXK3; HKIII; HK III
uniprot entry name :
HXK3_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE CDVSLIPSVD GGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA GVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC GAGVAAVVEK IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE DGSGKGAALV TAVACRLAQL TRV
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl pH8.0, 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Signal Transduction
products description :
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis. Recombinant human Hexokinase3, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Jon E. et al.,(2003) J.Exp Biology. 206 : 2049-2057. Furuta H. et al.,(1996) Genomics. 1996; 36(1):206-9.
ncbi gb acc num :
NM_002115.2
ncbi mol weight :
101.1kDa (943aa)
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway 82979!!Amino Sugar And Nucleotide Sugar Metabolism Pathway 350!!Butirosin And Neomycin Biosynthesis Pathway 145809!!Butirosin And Neomycin Biosynthesis Pathway 145786!!Carbohydrate Digestion And Absorption Pathway 170720!!Carbohydrate Digestion And Absorption Pathway 170654!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Central Carbon Metabolism In Cancer Pathway 1059538!!Central Carbon Metabolism In Cancer Pathway 1084231
ncbi summary :
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]