protein

Protein kinase Casein Kinase 2 alpha (PKCK2, 1-391aa) Human, Recombinant, His-tag, E Coli

MBS203045

0.05 mg

310 USD

Recombinant Protein

Protein kinase Casein Kinase 2 alpha (PKCK2, 1-391aa) Human, Recombinant, His-tag, E Coli

Protein kinase Casein Kinase 2 alpha

CSNK2A1; CK2A1; Protein kinase Casein Kinase 2 alpha; PKCK2; CK II; EC 2.7.11.1; Casein kinase II subunit alpha; Casein kinase II alpha 1 subunit isoform a Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1 subunit; Casein kinase II alpha subunit; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII.

casein kinase II subunit alpha isoform a; Casein kinase II subunit alpha; casein kinase II subunit alpha; CK II alpha 3; CK2 catalytic subunit alpha; casein kinase II alpha 1 polypeptide pseudogene; casein kinase II alpha 1 subunit; protein kinase CK2; casein kinase 2, alpha 1 polypeptide

CSNK2A1; CSNK2A1; CKII; CK2A1; CSNK2A3; CK2A1; CK II alpha

CSK21_HUMAN

391

MGSSHHHHHH SSGLVPRGSH MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MMSGISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q

> 90% by SDS - PAGE

Liquid. 20mM Tris pH8.0, 500mM NaCl, 1mM DTT, 50% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

Protein Kinase Casein Kinase 2 (PKCK2, also called CK2) is a ubiquitous Ser/Thr kinase expressed in all eukaryotes. PKCK2 is a tetramer composed of two catalytic kinase domains, alpha subunits, and two identical regulatory beta subunits. It has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm, and other cellular processes. Recombinant human PKCK2, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Kim YK., et al. (2006) J Biol Chem. 281(48):36752-7.

4503095

NP_001886

NM_001895.3

47.3 kDa (411 aa), confirmed by MALDI-TOF.

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha-synuclein Signaling Pathway (137913); Axon Guidance Pathway (105688); BCR Signaling Pathway (138058); BDNF Signaling Pathway (712093); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Condensation Of Prometaphase Chromosomes Pathway (730316); Developmental Biology Pathway (477129)

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2014]

CK2A1: an ubiquitous protein kinase of the CK2 family. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. The isoforms are rarely specified in publications. Two splice variant isoforms have been found. Participates in Wnt signaling. Phosphorylates E2 ubiquitin conjugating enzyme UBC3B inducing its interaction with beta-TRCp and enhancing beta-catenin degradation. May control IkB-alpha and p27Kip1 degradation. component of a CK2-SPT16-SSRP1 complex composed of SSRP1, PT16 CK2-A1, CK2-A2 and CK2-B the complex associating following UV irradiation. Interacts with RNPS1. Mouse transgene causes mammary gland hyperplasia and lymphoma, and activation by bovine parasites leads to fatal lymphoproliferation. Expression and activity are elevated in lung tumors and breast tumors. Antisense drives apoptosis of tumor cell lines and xenografts. Involved in DNA break repair by phosphorylation of scaffold protein XRCC1, phosphorylation of BRCA1, and phosphorylation of p53 in response to UV irradiation. Drosophila CK2 (Timekeeper) is involved in circadian regulation. Phosphorylates and binds to a major component of the inclusion bodies seen in Parkinson?s patients. Inhibitors: 4,5,6,7-tetrabromobenzotriazole (TBB). Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; CK2 family. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: Sin3 complex; plasma membrane; PcG protein complex; NuRD complex; nucleus; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; protein N-terminus binding; Hsp90 protein binding; ATP binding. Biological Process: axon guidance; Wnt receptor signaling pathway; transcription, DNA-dependent; rhythmic process; negative regulation of caspase activity; signal transduction; positive regulation of cell growth; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of protein catabolic process; mitotic cell cycle; positive regulation of Wnt receptor signaling pathway

0.05 mg

310 USD

0.25 mg

720