protein

His-PP2Calpha(His-tag Protein phosphatase 2C), Human, Recombinant, E Coli

MBS203042

0.1 mg

255 USD

Recombinant Protein

His-PP2Calpha(His-tag Protein phosphatase 2C), Human, Recombinant, E Coli

His-PP2Calpha

PDP1; PDP; PPM2C; PP2C alpha; Protein phosphatase 2C; PDP 1; EC 3.1.3.43; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1; Pyruvate dehydrogenase acetyl-transferring-phosphatase 1; PPM1A; Protein phosphatase 1A (formerly 2C); ppm1a; FLJ42306; MGC9201; PP2CA; PP2Calpha; PP2C-alpha; protein phosphatase Mg2+/Mn2+ dependent 1A

protein phosphatase 1A isoform 1; Protein phosphatase 1A; protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA

PPM1A; PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA; PPPM1A; PP2C-alpha

PPM1A_HUMAN

382

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW

> 95% by SDS - PAGE

Liquid. In 10 mM Tris-HCl, pH7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20%glycerol.

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: >8,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2. Pre-warm reaction mix in heat block at 37C . 3. Add recombinant PP2C alpha solution with various concentrations (2ug, 3ug, 4ug) in 200ul reaction buffer. 4. Mix by inversion and Incubate at 37C for 10minutes. 5. Stop the reaction by adding the 800ul of 0.1N NaOH. 6. Record the absorbance at 405nm

Signal Transduction

Protein phosphatase 2C(PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

Das, A. K. et al. (1996) EMBO J. 15, 6798-6809

10337595

NP_066283

NM_021003.4

46.6 kDa (418 aa), confirmed by MALDI-TOF.

BMP Receptor Signaling Pathway 137948!!Disease Pathway 530764!!Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway 645266!!Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway 160972!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!IGF1R Signaling Cascade Pathway 730346!!IRS-mediated Signalling Pathway 106426!!IRS-related Events Pathway 106424!!IRS-related Events Triggered By IGF1R Pathway 730348

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

0.1 mg

255 USD