protein

SHP-1,243-541 aa

MBS203041

0.1 mg

375 USD

Recombinant Protein

SHP-1,243-541 aa

SHP-1

PTPN6; HCP; PTP1C; Tyrosine-protein phosphatase non-receptor type 6; EC 3.1.3.48; Protein-tyrosine phosphatase 1C; PTP-1C; Hematopoietic cell protein-tyrosine phosphatase; SH-PTP1; Protein-tyrosine phosphatase SHP-1; PTPN6; HCP; HCPH; SHP1; HPTP1C; SHP-1L EC 3.1.3.48; Protein tyrosine phosphatase non-receptor type 6 isoform 1; 70 kda SHP1L protein; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; HPTP 1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase SHP 1; Protein tyrosine phosphatase SHP1; PTP 1C; PTPN 6; SH PTP 1; SH PTP1; SHP 1; SHP 1L; SHP1L; SHPTP 1; SHPTP1; Tyrosine protein phosphatase non receptor type 6

tyrosine-protein phosphatase non-receptor type 6 isoform 1; Tyrosine-protein phosphatase non-receptor type 6; tyrosine-protein phosphatase non-receptor type 6; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP-1; protein tyrosine phosphatase, non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1

SHP-1

PTPN6; PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1; HCP; PTP1C; PTP-1C

PTN6_HUMAN

595

MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY

> 95% by SDS - PAGE

Liquid. 25 mM Tris-HCl, pH7.5, 2 mM beta-mercaptoethanol, 1 mM EDTA. 1mMDTT, 20%Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: >5,000units/mg of SHP-1. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: One unit will hydrolyze 1nanomole of p-nitrophenyl- . phosphatate(pNPP) per minute at pH 7.4 at 37degree Celcius using 10mM of substrate.

Signal Transduction

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus.

Shen SH., et al. (1991) Nature 352, 736-739. Wu C., et al. (2003) Gene. 306,1-12.

18104989

NP_002822

NM_002831.5

34.3 kDa (300 aa), confirmed by MALDI-TOF.

Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); CXCR4-mediated Signaling Events Pathway (137910); Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

SHP-1: an SH2-containing ubiquitously expressed tyrosine-specific protein phosphatase. Plays a key role in hematopoiesis. Directly links growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Three alternatively spliced isoforms have been described. Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor). Chromosomal Location of Human Ortholog: 12p13. Cellular Component: membrane; cytoplasm; nucleolus; alpha-beta T cell receptor complex; intercellular junction; nucleus; cytosol. Molecular Function: protein binding; phosphotyrosine binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; SH2 domain binding; protein kinase binding; SH3 domain binding. Biological Process: negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid dephosphorylation; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of cell proliferation; B cell receptor signaling pathway; negative regulation of T cell proliferation; negative regulation of B cell receptor signaling pathway; positive regulation of cell proliferation; abortive mitotic cell cycle; cell differentiation; negative regulation of peptidyl-tyrosine phosphorylation; natural killer cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; positive regulation of cell adhesion mediated by integrin; negative regulation of T cell receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; positive regulation of phosphoinositide 3-kinase cascade; cell proliferation; G-protein coupled receptor protein signaling pathway; T cell costimulation; regulation of B cell differentiation; platelet formation; blood coagulation; leukocyte migration

0.1 mg

375 USD

0.5 mg

925