protein

PTP-1B(Protein Tyrosine Phosphatase1B), Human, Recombinant, E Coli

MBS203040

0.1 mg

255 USD

Recombinant Protein

PTP-1B(Protein Tyrosine Phosphatase1B), Human, Recombinant, E Coli

PTP-1B

PTPN1; PTP1B; PTP-1B; Protein Tyrosine Phosphatase 1B; EC 3.1.3.48; Protein-tyrosine phosphatase1B; Tyrosine-protein phosphatase non-receptor type 1; Protein tyrosine phosphatase non-receptor type 1; Protein Tyrosine Phosphatase Non Receptor Type-1

tyrosine-protein phosphatase non-receptor type 1 isoform 1; Tyrosine-protein phosphatase non-receptor type 1; tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B

PTP-1B

PTPN1; PTPN1; PTP1B; PTP1B; PTP-1B

PTN1_HUMAN

435

MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N

> 95% by SDS - PAGE

Liquid. 25 mM Tris-HCl, pH7.5, 2 mM beta-mercaptoethanol, 1 mM EDTA.1mMDTT, 20%Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity: 6,000-12,000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. Assay: 1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2. Pre-warm reaction mix in heat block at 37C . 3. Add recombinant PTP1B solution with various concentrations (2ug, 3ug, 4ug) in 200ul reaction buffer. 4. Mix by inversion and Incubate at 37C for 10minutes. 5. Stop the reaction by adding the 800ul of 0.1N NaOH. 6. Record the absorbance at 405nm.

Signal Transduction

The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.

Chernoff J., et al. (1990) Proc. Natl. Acad. Sci. uSA. 87, 2735-9

4506289

NP_002818

NM_002827.3

37.3 kDa (321 aa)

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cytokine Signaling In Immune System Pathway (366171); EGF Receptor (ErbB1) Signaling Pathway (137970); Growth Hormone Receptor Signaling Pathway (477128); Hemostasis Pathway (106028); IGF1 Pathway (137992); Immune System Pathway (106386); Insulin Pathway (138026)

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes. Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Phosphatase. Chromosomal Location of Human Ortholog: 20q13.1-q13.2. Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol. Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding. Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; regulation of endocytosis; actin cytoskeleton reorganization; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; regulation of signal transduction; blood coagulation. Disease: Diabetes Mellitus, Noninsulin-dependent

0.1 mg

255 USD

0.5 mg

535