Hsp27, Human, Recombinant, E Coli | MyBioSource MBS203034 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Hsp27, Human, Recombinant, E Coli

catalog :

MBS203034

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203034

products type :

Recombinant Protein

products full name :

Hsp27, Human, Recombinant, E Coli

products short name :

Hsp27

products name syn :

Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.

other names :

heat shock protein beta-1; Heat shock protein beta-1; heat shock protein beta-1; 28 kDa heat shock protein; HSP 27; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

products gene name :

Hsp27

other gene names :

HSPB1; HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; HSP27; HSP28; HspB1; HSP 27; SRP27

uniprot entry name :

HSPB1_HUMAN

sequence length :

205

sequence :

MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 100 mM KCl, 1 mM DTT

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Heat Shock Proteins

products description :

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.

products references :

Ehrnsperger M.,et al. (1997) EMBO J. 16, 221-229. Butt E., et al. (2001) J. Biol. Chem. 276, 7108-7113.

ncbi gi num :

4504517

ncbi acc num :

NP_001531

ncbi gb acc num :

NM_001540.3

ncbi mol weight :

22.7kDa (205aa), confirmed by MALDI-TOF.

ncbi pathways :

AUF1 (hnRNP D0) Destabilizes MRNA Pathway (187212); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937); IL-3 Signaling Pathway (198881); IL-6 Signaling Pathway (198864); MAPK Signaling Pathway (198779)

ncbi summary :

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

uniprot summary :

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. Protein type: Motility/polarity/chemotaxis; Heat shock protein; Chaperone. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; nucleus; cytosol; Z disc. Molecular Function: protein kinase C inhibitor activity; identical protein binding; ubiquitin binding; protein binding; protein kinase C binding; protein kinase binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; retinal homeostasis; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of apoptosis; regulation of translational initiation. Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage