product summary
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company name :
MyBioSource
product type :
protein
product name :
DnaJ(Amino acids 1-376), E Coli, Recombinant, E Coli
catalog :
MBS203031
quantity :
0.1 mg
price :
255 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203031
products type :
Recombinant Protein
products full name :
DnaJ(Amino acids 1-376), E Coli, Recombinant, E Coli
products short name :
DnaJ
products name syn :
DNAJA1; DNAJ2; HDJ2; HSJ2; HSPF4; DnaJ (1-376aa); Heat shock 40 kDa protein 4; DnaJ protein homolog 2; HDJ-2; HSJ-2; HSDJ; DnaJ homolog subfamily A member 1
other names :
chaperone Hsp40, DnaK co-chaperone; Chaperone protein DnaJ; chaperone Hsp40, DnaK co-chaperone; HSP40; Heat shock protein J
products gene name :
DnaJ
other gene names :
dnaJ; dnaJ; ECK0015; faa; groP; grpC; JW0014; groP
uniprot entry name :
DNAJ_ECOLI
sequence length :
376
sequence :
MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR
purity :
> 95% by SDS - PAGE
form :
Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5mM DTT,10%Glycerol.
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli
other info2 :
Expression System: E Coli
products categories :
Heat Shock Proteins
products description :
DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. DnaJ(amino acids 1-376) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.
products references :
Bardwell,J.C., et al (1986) J.Biol.Chem.261(4);1782-1785. Ohki M.,et al (1986) J.Biol.Chem.261(4);1778-1781. Zylicz M., et.al.(1989) EMBO J 8(5)1601-8 . Landry SJ., et al. (2003) Biochemistry 42(17)4926-36.
ncbi gi num :
16128009
ncbi acc num :
NP_414556
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P08622
ncbi mol weight :
41.1kDa (376aa), confirmed by MALDI-TOF.
ncbi summary :
DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. [More information is available at EcoGene: EG10240]. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system. [More information is available at EcoCyc: EG10240].
size1 :
0.1 mg
price1 :
255 USD
size2 :
0.5 mg
price2 :
535
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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