catalog number :
MBS2030194
products type :
Recombinant Protein
products full name :
Recombinant Nidogen (NID)
products short name :
Nidogen
other names :
nidogen; Nidogen-1; nidogen-1; nidogen 1; Entactin
other gene names :
NID1; NID1; NID; NID; NID-1
uniprot entry name :
NID1_HUMAN
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AFLHVPAKVI IGLAFDCVDK MVYWTDITEP SIGRASLHGG EPTTIIRQDL GSPEGIAVDH LGRNIFWTDS NLDRIEVAKL DGTQRRVLFE TDLVNPRGIV TDSVRGNLYW TDWNRDNPKI ETSYMDGTNR RILVQDDLGL PNGLTFDAFS SQLCWVDAGT NRAECLNPSQ PSRRKALEGL QYPFAVTSYG KNLYFTDWKMNSVVALDLAI SKETDAFQPH KQTRLYGITT ALSQCPQGHN YCSVNNGGC
form :
Supplied as lyophilized form in 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Predicted isoelectric point: 6.3. Predicted Molecular Mass: 31.6kDa. Endotoxin: <1.0EU per 1ug (determined by the LAL method). Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane.
other info2 :
Expression System: Prokaryotic expression. Tag: two N-terminal Tags, His-tag and T7-tag. Organism Species: Homo sapiens (Human). Fragment: Ala971~Cys1219. Usage: Reconstitute in sterile ddH2O.
ncbi acc num :
AAA59932.1
ncbi pathways :
Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244); Laminin Interactions Pathway (1270253)
ncbi summary :
This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]
uniprot summary :
Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: extracellular matrix; extracellular region; basement membrane; basal lamina. Molecular Function: laminin-1 binding; collagen binding; proteoglycan binding; laminin binding; calcium ion binding. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell-matrix adhesion; glomerular basement membrane development