protein

DJ-1(PARK7),Human, Recombinant, His-tag, E Coli

MBS203016

0.1 mg

310 USD

Recombinant Protein

DJ-1(PARK7),Human, Recombinant, His-tag, E Coli

DJ-1

PARK7; DJ-1 (PARK7); Oncogene DJ1; Parkinson disease protein 7; Protein DJ-1; Parkinson disease protein 7 CAP1; DJ1; DJ1 protein; Oncogene DJ1; SP22.

protein DJ-1; Protein DJ-1; protein DJ-1; Parkinson disease (autosomal recessive, early onset) 7; epididymis secretory sperm binding protein Li 67p; oncogene DJ1; parkinson protein 7; Oncogene DJ1; Parkinson disease protein 7

DJ-1

PARK7; PARK7; DJ1; DJ-1; HEL-S-67p

PARK7_HUMAN

189

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK RALVILAKGA EEMETVIPVD VMRRAGIKVT VAGLAGKDPV QCSRDVVICP DASLEDAKKE GPYDVVVLPG GNLGAQNLSE SAAVKEILKE QENRKGLIAA ICAGPTALLA HEIGFGSKVT THPLAKDKMM NGGHYTYSEN RVEKDGLILT SRGPGTSFEF ALAIVEALNG KEVAAQVKAP LVLKD

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl pH8.0, 20% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Neuroscience

The DJ-1 is a ubiquitously expressed protein involved in various cellular processes including spermatogenesis and fertilization, cancer, RNA-binding, androgen-receptor signaling and oxidative stress. Mutations in the DJ-1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (Park7). Recombinant human DJ-1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Choi J., et al. (2006) J. Biol Chem. 281(16):10816-24. Maraganore DM., et al. (2004) Neurology. 63(3):550-3.

31543380

NP_009193

NM_007262.4

24 kDa (225 aa), confirmed by MALDI-TOF.

Alpha-synuclein Signaling Pathway (137913); Androgen Receptor Signaling Pathway (198806); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Synaptic Vesicle Pathway (672457)

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration. Protein type: Tumor suppressor; Transcription regulation; EC 3.4.-.-; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 1p36.23. Cellular Component: PML body; mitochondrion; mitochondrial matrix; axon; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; chromatin; cytosol; mitochondrial respiratory chain complex I; lipid raft. Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; peptidase activity; mRNA binding; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; kinase binding; superoxide dismutase copper chaperone activity; single-stranded DNA binding; receptor binding. Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of peptidyl-serine phosphorylation; negative regulation of protein sumoylation; methylglyoxal catabolic process to D-lactate; positive regulation of protein kinase B signaling cascade; membrane depolarization; detoxification of mercury ion; Ras protein signal transduction; regulation of inflammatory response; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; autophagy; lactate biosynthetic process; negative regulation of protein kinase activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of apoptosis. Disease: Parkinson Disease 7, Autosomal Recessive Early-onset

0.1 mg

310 USD

0.5 mg

720