catalog number :
MBS2030070
products type :
Recombinant Protein
products full name :
Recombinant NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)
products short name :
NLR Family
products gene name :
NLRP3
host :
Host: E Coli. Source: Prokaryotic expression
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. CHRVKTLSLG FFHNSPKEEE EERRGGRPLD QVQCVFPDTH VACSSRLVNC CLTSSFCRGL FSSLSTNRSL TELDLSDNTL GDPGMRVLCE ALQHPGCNIQ RLWLGRCGLS HQCCFDISSV LSSSQKLVEL DLSDNALGDF GIRLLCVGLK HLLCNLQKLW LVSCCLTSAC CQDLALVLSS NHSLTRLYIG ENALGDSGVQ VLCEKMKDPQ CNLQKLGLVN SGLTSICCSA LTSVLKTNQN FTHLYLRSNA LGDTGLRLLC EGLLHPDCKL QMLELDNCSL TSHSCWNLST ILTHNHSLRK LNLGNNDLGD LCVVTLCEVL KQQGCLLQSL QLGEMYLNRE TKRALEALQE EKPELTIVFEISW
form :
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Predicted isoelectric point: 6.1. Predicted Molecular Mass: 43.9kDa. Endotoxin: <1.0EU per 1ug (determined by the LAL method)
other info2 :
Expression System: Prokaryotic expression. Tag: Two N-terminal Tags, His-tag and T7-tag. Organism Species: Mus musculus (Mouse). Fragment: Cys671~Trp1033. Usage: Reconstitute in sterile ddH2O. Accession: T1W2H6