Recombinant S-Phase Kinase Associated Protein 2 (SKP2) | MyBioSource MBS2030002 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant S-Phase Kinase Associated Protein 2 (SKP2)

catalog :

MBS2030002

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2030002

products type :

Recombinant Protein

products full name :

Recombinant S-Phase Kinase Associated Protein 2 (SKP2)

products short name :

[S-Phase Kinase Associated Protein 2]

other names :

[S-phase kinase associated protein 2, partial; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2, E3 ubiquitin protein ligase; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2]

products gene name :

[SKP2]

other gene names :

[SKP2; SKP2; p45; FBL1; FLB1; FBXL1; FBXL1]

uniprot entry name :

SKP2_HUMAN

host :

E.coli

purity :

> 90%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01%skl, 5% Trehalose and Proclin300.

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Lys43~Ala397. Tags: N-terminal His Tag. Subcellular Location: Nucleus, Cytoplasm. Traits: Freeze-dried powder. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

other info2 :

Predicted isoelectric point: 6.1. Predicted Molecular Mass: 43.7KDa. Accurate Molecular Mass: 41kDa as determined by SDS-PAGE reducing conditions.

ncbi gi num :

68161558

ncbi acc num :

AAY87142.1

ncbi mol weight :

23,763 Da

ncbi pathways :

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (1269838); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (1269851); Adaptive Immune System Pathway (1269171); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); C-MYC Pathway (169344); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011]

uniprot summary :

SKP2: an F-box protein of the SCF (SKP1-CUL1-F- box protein) E3 ubiquitin ligase system. The F-box component of the SCF complex is responsible for recognizing different substrates for ubiquitination. The SCF E3 complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Three alternatively-spliced isoforms have been described. Protein type: Ubiquitin conjugating system; Cell cycle regulation. Chromosomal Location of Human Ortholog: 5p13. Cellular Component: nucleoplasm; cytoplasm; nucleolus; SCF ubiquitin ligase complex; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity. Biological Process: regulation of apoptosis; cell proliferation; protein polyubiquitination; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of cell cycle; positive regulation of smooth muscle cell proliferation; positive regulation of estrogen receptor signaling pathway; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

265

size3 :

0.1 mg

price3 :

410

size4 :

0.2 mg

price4 :

500

size5 :

0.5 mg

price5 :

975

size6 :

1 mg

price6 :

1440

more info or order :

MyBioSource product webpage