catalog number :
MBS2029991
products type :
Recombinant Protein
products full name :
Recombinant Microtubule Associated Protein 4 (MAP4)
products short name :
[Microtubule Associated Protein 4]
other names :
[microtubule-associated protein 4; Microtubule-associated protein 4]
products gene name :
[MAP4]
other gene names :
[MAP4; MAP-4]
uniprot entry name :
MAP4_HUMAN
reactivity :
Mus musculus (Mouse)
form :
100mM NaHCO 3 , 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
Sequence Information
image2 heading :
SDS-Page
image3 heading :
Testing Data
other info1 :
Source: Prokaryotic expression. Residues: Thr882~Glu1062. Tags: N-terminal His-Tag. Tissue Specificity: Kidney, Brain. Subcellular Location: Cytoplasm, cytoskeleton. Traits: Freeze-dried powder
other info2 :
Predicted isoelectric point: 10.2. Predicted Molecular Mass: 22.3kDa. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3.Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 100mM NaHCO 3 , 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ncbi acc num :
AAA67361.1
uniprot summary :
MAP4: a microtuble-associated protein detected predominately in nonneuronal cells. May play a role in microtubule stabilization as well as promoting microtubule assembly. Four alternatively spliced variants have been described. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: microtubule cytoskeleton; microtubule; microtubule associated complex; cytoplasm; plasma membrane. Molecular Function: protein binding; microtubule binding; structural molecule activity. Biological Process: mitotic spindle organization and biogenesis; cell division; microtubule sliding; establishment of spindle orientation