protein

Recombinant Prothrombin Fragment 1+2 (F1+2)

MBS2029665

0.01 mg

150 USD

Recombinant Protein

Recombinant Prothrombin Fragment 1+2 (F1+2)

[Prothrombin Fragment 1+2]

[F1+2]

E. coli

Rat

>95%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

Original Concentration: 200µg/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Source: Prokaryotic expression. Residues: Ala44~Arg323. Tags: N-terminal His-Tag. Tissue Specificity: Liver. Traits: Freeze-dried powder. Predicted isoelectric point: 5.1. Predicted Molecular Mass: 35.4kDa. Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

0.01 mg

150 USD

0.05 mg

290

0.1 mg

455

0.2 mg

560

0.5 mg

1080

1 mg

1610