Recombinant Serine/Arginine Rich Splicing Factor 2 (SRSF2) | MyBioSource MBS2029592 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Serine/Arginine Rich Splicing Factor 2 (SRSF2)

catalog :

MBS2029592

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2029592

products type :

Recombinant Protein

products full name :

Recombinant Serine/Arginine Rich Splicing Factor 2 (SRSF2)

products short name :

[Serine/Arginine Rich Splicing Factor 2]

other names :

[serine/arginine-rich splicing factor 2; Serine/arginine-rich splicing factor 2; serine/arginine-rich splicing factor 2; serine/arginine-rich splicing factor 2; Protein PR264; Splicing component, 35 kDa; Splicing factor SC35; SC-35; Splicing factor, arginine/serine-rich 2]

products gene name :

[SRSF2]

other gene names :

[SRSF2; SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b; SFRS2; SC-35]

uniprot entry name :

SRSF2_HUMAN

host :

E.coli

purity :

>90%

form :

100mM NaHCO 3 , 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

concentration :

200ug/ml

storage stability :

Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residues: Thr14~Ser221. Tags: Two N-terminal Tags, His-tag and GST-tag. Tissue Specificity: Bone marrow. Subcellular Location: Nucleus, nucleoplasm, Nucleus speckle. Traits: Freeze-dried powder.

other info2 :

Predicted isoelectric point: 11.9. Predicted Molecular Mass: 54.1kDa. Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 100mM NaHCO 3 , 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

306482646

ncbi acc num :

NP_001182356.1

ncbi gb acc num :

NM_001195427.1

ncbi mol weight :

24,153 Da

ncbi pathways :

Cleavage Of Growing Transcript In The Termination Region Pathway (1269686); Gene Expression Pathway (1269649); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688); RNA Polymerase II Transcription Pathway (1269675); RNA Polymerase II Transcription Termination Pathway (1269685); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Transport Of Mature Transcript To Cytoplasm Pathway (1269693)

ncbi summary :

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010]

uniprot summary :

SFRS2: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5 - and 3 -splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5 - and 3 -splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5 -AGSAGAGTA-3 (S=C or G) or 5 -GTTCGAGTA-3 . Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Belongs to the splicing factor SR family. Protein type: RNA splicing; Spliceosome; RNA processing. Chromosomal Location of Human Ortholog: 17q25.1. Cellular Component: nucleoplasm; spliceosome; PML body; cytoplasm; nuclear speck; nucleus. Molecular Function: protein binding; nucleotide binding; transcription corepressor activity. Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3 -end processing; mRNA processing; termination of RNA polymerase II transcription

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

265

size3 :

0.1 mg

price3 :

410

size4 :

0.2 mg

price4 :

500

size5 :

0.5 mg

price5 :

975

size6 :

1 mg

price6 :

1440

more info or order :

MyBioSource product webpage