Recombinant Glutamate Carboxypeptidase II (GCPII) | MyBioSource MBS2029572 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Glutamate Carboxypeptidase II (GCPII)

catalog :

MBS2029572

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2029572

products type :

Recombinant Protein

products full name :

Recombinant Glutamate Carboxypeptidase II (GCPII)

products short name :

[Glutamate Carboxypeptidase II]

other names :

[Glutamate carboxypeptidase 2; Glutamate carboxypeptidase 2; glutamate carboxypeptidase 2; folate hydrolase (prostate-specific membrane antigen) 1; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; FGCP; Glutamate carboxypeptidase II; GCPII; Membrane glutamate carboxypeptidase; mGCP; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; PSM; PSMA; Pteroylpoly-gamma-glutamate carboxypeptidase]

products gene name :

[GCPII]

other gene names :

[FOLH1; FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase; FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA]

uniprot entry name :

FOLH1_HUMAN

host :

E.coli

sequence :

ANEYAYR RGIAEAVGLP SIPVHPIGYY DAQKLLEKMG GSAPPDSSWR GSLKVPYNVG PGFTGNFSTQ KVKMHIHSTN EVTRIYNVIG TLRGAVEPDR YVILGGHRDS WVFGGIDPQS GAAVVHEIVR SFGTLKKEGW RPRRTILFAS WDAEEFGLLG STEWAEENSR LLQERGVAYI NADSSIEGNY TLRVDCTPLM YSLVHNLTKE LKSPDEGFEG KSLYESWTKK SPSPEFSGMP RISKLGSGND FEVFFQRLGI ASGRARYTKN WETNKFSGYP LYHSVYETYE LVEKFYDPMFKYHLTVAQVR GGMVFEL

purity :

>95%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

concentration :

Original Concentration: 200 ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

app notes :

(May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Ala274~Leu587. Tags: N-terminal His Tag. Subcellular Location: Membrane. Traits: Freeze-dried powder. Predicted isoelectric point: 7.3. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

548615

ncbi acc num :

Q04609.1

ncbi mol weight :

Predicted Molecular Mass: 39.1kDa. Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Metabolic Pathways (132956); One Carbon Metabolism Pathway (198756); Vitamin Digestion And Absorption Pathway (199556); Vitamin Digestion And Absorption Pathway (199538)

ncbi summary :

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

uniprot summary :

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Protease; EC 3.4.17.21. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: membrane; integral to plasma membrane; cytoplasm. Molecular Function: peptidase activity; dipeptidase activity; carboxypeptidase activity; metallopeptidase activity; metal ion binding. Biological Process: folic acid and derivative metabolic process; proteolysis

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

260

size3 :

0.1 mg

price3 :

400

size4 :

0.2 mg

price4 :

495

size5 :

0.5 mg

price5 :

955

size6 :

1 mg

price6 :

1415

more info or order :

MyBioSource product webpage