Recombinant Adaptor Protein Phosphotyrosine Interaction PH Domain And leucine Zipper Containing Protein 1 (APPL1) | MyBioSource MBS2029487 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Adaptor Protein Phosphotyrosine Interaction PH Domain And leucine Zipper Containing Protein 1 (APPL1)

catalog :

MBS2029487

quantity :

0.01 mg

price :

145 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

Sequence

product information

catalog number :

MBS2029487

products type :

Recombinant Protein

products full name :

Recombinant Adaptor Protein Phosphotyrosine Interaction PH Domain And leucine Zipper Containing Protein 1 (APPL1)

products short name :

[Adaptor Protein Phosphotyrosine Interaction PH Domain And leucine Zipper Containing Protein 1]

other names :

[Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; DCC-interacting protein 13-alpha; DCC-interacting protein 13-alpha; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; Adapter protein containing PH domain, PTB domain and leucine zipper motif 1]

products gene name :

[APPL1]

other gene names :

[APPL1; APPL1; APPL; DIP13alpha; ; Dip13-alpha]

uniprot entry name :

DP13A_HUMAN

host :

E Coli

purity :

>90%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

concentration :

Original Concentration: 200 ug/mL

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), Positive Control, Immunogen. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

image2 heading :

Testing Data

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Phe404~Ser673. Tags: N-terminal His Tag. Subcellular Location: Membrane, Nucleus. Traits: Freeze-dried powder. Predicted isoelectric point: 6.2. Predicted Molecular Mass: 33.7kDa. Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions. Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

20306914

ncbi acc num :

AAH28599.1

ncbi pathways :

Apoptosis Pathway (1270262); Caspase Activation Via Extrinsic Apoptotic Signalig Pathway (1270263); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Coregulation Of Androgen Receptor Activity Pathway (138085); EGFR1 Signaling Pathway (198782); FSH Signaling Pathway (672455); Ligand-independent Caspase Activation Via DCC Pathway (1270267); Pathways In Cancer (83105); Programmed Cell Death Pathway (1270261)

ncbi summary :

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]

uniprot summary :

APPL: an effector protein of the small GTPase Rab5, a key regulator of endocytosis. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Contains a PH domain and an N-terminal PID domain. Its interaction with RAB5A/Rab5 is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Phosphorylated upon DNA damage. Protein type: Nuclear receptor co-regulator; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 3p14.3. Cellular Component: early endosome membrane; cytoplasm; endosome membrane; vesicle membrane; NuRD complex; cytosol; nucleus. Molecular Function: protein kinase B binding; identical protein binding; protein binding. Biological Process: cell proliferation; positive regulation of apoptosis; insulin receptor signaling pathway; regulation of glucose import; cell cycle; signal transduction. Disease: Maturity-onset Diabetes Of The Young, Type 14

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

275

size3 :

0.1 mg

price3 :

430

size4 :

0.2 mg

price4 :

525

size5 :

0.5 mg

price5 :

1015

size6 :

1 mg

price6 :

1515

more info or order :

MyBioSource product webpage