catalog number :
MBS2029337
products type :
Recombinant Protein
products full name :
Recombinant Glutamate Cysteine Ligase, Modifier Subunit (GCLM)
products short name :
[Glutamate Cysteine Ligase]
other names :
[glutamate-cysteine ligase; Glutamate--cysteine ligase, chloroplastic; glutamate-cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Protein ROOT MERISTEMLESS 1; AtGCL; Protein cadmium-sensitive 2; Protein phytoalexin-deficient 2]
products gene name :
[GCLM]
other gene names :
[GSH1; GSH1; ATECS1; CAD2; CADMIUM SENSITIVE 2; F7H19.290; F7H19_290; GAMMA-GLUTAMYL-CYSTEINE SYNTHETASE; GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; glutamate-cysteine ligase; GLUTAMATE-CYSTEINE LIGASE; GSH1; GSHA; PAD2; PHYTOALEXIN DEFICIENT 2; RML1; ROOT MERISTEMLESS 1; CAD2; GCL; PAD2; RML1; GCS; AtGCL]
uniprot entry name :
GSH1_ARATH
concentration :
Original concentration: 200 ug/mL
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
tested application :
Positive Control, Immunogen, SDS-PAGE,WB. (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
SDS-Page
other info1 :
Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Ser40~Ser251. Tags: N-terminal His Tag. Traits: Freeze-dried powder. Buffer: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
other info2 :
Predicted isoelectric point: 4.9. Predicted Molecular Mass: 27.6kDa. Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ncbi acc num :
NP_194041.1
ncbi gb acc num :
NM_118439.3
ncbi pathways :
Glutathione Biosynthesis, Glutamate = Glutathione Pathway (382710); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (468307); Glutathione Metabolism Pathway (4032); Glutathione Metabolism Pathway (343); Metabolic Pathways (132014)
ncbi summary :
Encodes the enzyme glutamate-cysteine ligase catalyzing the first, and rate-limiting, step of glutathione biosynthesis. Required for cell proliferation at the root tip. Involved in susceptibility to the bacterial pathogen Pseudomonas syringae. Mutants are phytoalexin defective.
uniprot summary :
Seems to play an important role in controlling the expression of resistance responses like the regulation of salicylic acid (SA) and phytoalexin (camalexin) production. Involved in resistance to fungal and bacterial pathogens. Required for the regulation of cell proliferation in root apical meristems through the GSH-dependent developmental pathway. Also participates in the detoxification process, the antioxidant response and is essential for embryo development and proper seed maturation.