protein

Recombinant Apelin (APLN)

MBS2028954

0.01 mg

145 USD

Recombinant Protein

Recombinant Apelin (APLN)

[Apelin]

[apelin preproprotein; Apelin; apelin; apelin; APJ endogenous ligand]

[APLN]

[APLN; APLN; APEL; XNPEP2; APEL]

APEL_HUMAN

E Coli

77

> 97%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Organism Species: Mus musculus (Mouse). Source: Prokaryotic expression. Residues: Val23~Phe77. Tags: N-terminal His and GST Tag. Subcellular Location: Secreted, Extracellular matrix. Traits: Freeze-dried powder

Predicted isoelectric point: 8.8. Predicted Molecular Mass: 36.2kDa. Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

45120090

NP_059109.3

NM_017413.4

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543)

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.[provided by RefSeq, Feb 2010]

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xq25. Cellular Component: extracellular space; extracellular region. Molecular Function: G-protein-coupled receptor binding; hormone activity; apelin receptor binding; receptor binding. Biological Process: lactation; immune response; signal transduction

0.01 mg

145 USD

0.05 mg

270

0.1 mg

425

0.2 mg

520

0.5 mg

1005

1 mg

1500