catalog number :
MBS2028731
products full name :
Polyclonal Antibody to Calretinin (CR)
products short name :
[Calretinin]
other names :
[calretinin isoform 1; Calretinin; calretinin; calbindin 2; 29 kDa calbindin]
products gene name :
[CR]
other gene names :
[CALB2; CALB2; CR; CAL2; CAB29; CAB29; CR]
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP.
app notes :
Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Immunohistochemistry (IHC)
image4 heading :
Immunohistochemistry (IHC)
image4 description :
DAB staining on IHC-P; Samples: Human Brain Tissue
image5 heading :
Immunohistochemistry (IHC)
image5 description :
DAB staining on IHC-P; Samples: Human Stomach Tissue
other info1 :
Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant Calretinin (Phe28~Met248 (Accession # P22676)) expressed in E.coli
other info2 :
Conjugate: No Conjugate. Immunogen: Recombinant calretinin (Phe28~Met248) expressed in E Coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2046032
ncbi acc num :
NP_001731.2
ncbi gb acc num :
NM_001740.4
ncbi summary :
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
uniprot summary :
CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: cytosol; gap junction; neuron projection; nucleus; synapse; terminal bouton. Molecular Function: calcium ion binding. Biological Process: regulation of cytosolic calcium ion concentration