Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) | MyBioSource MBS2028695 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)

catalog :

MBS2028695

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

HRP

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot; Sample: Recombinant GPX3, Human.

image 2 :

Western Blot;. Sample: Human Lung lysate;. Primary Ab: 1ug/ml Rabbit Anti-Human GPX3 Antibody. Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)

image 3 :

DAB staining on IHC-P;. Samples: Human Kidney Tissue;. Primary Ab: 10ug/ml Rabbit Anti- Human GPX3 Antibody. Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)

product information

catalog number :

MBS2028695

products type :

Antibody

products full name :

Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)

products short name :

[Glutathione Peroxidase 3]

other names :

[glutathione peroxidase 3 isoform 2; Glutathione peroxidase 3; glutathione peroxidase 3; glutathione peroxidase 3; Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P]

products gene name :

[GPX3]

other gene names :

[GPX3; GPX3; GPx-P; GSHPx-3; GSHPx-P; GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P]

clonality :

polyclonal

host :

rabbit

sequence length :

226

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2ug/mL;. Immunohistochemistry: 5-20ug/mL;. Immunocytochemistry: 5-20ug/mL;. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant GPX3 (Gln21~Tyr72) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2069320

ncbi gi num :

1048328959

ncbi acc num :

NP_001316719.1

ncbi gb acc num :

NM_001329790.1

uniprot acc num :

P22352

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Cellular Responses To Stress Pathway (1270414); Detoxification Of Reactive Oxygen Species Pathway (1270420); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (343); Glutathione Metabolism Pathway (198824); Metapathway Biotransformation (198837); NRF2 Pathway (1458227)

ncbi summary :

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

uniprot summary :

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family. Protein type: EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Oxidoreductase; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: extracellular region; extracellular space. Molecular Function: glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding. Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

340

size6 :

1 mg

price6 :

800

more info or order :

MyBioSource product webpage