catalog number :
MBS2028676
products full name :
Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)
products short name :
[Cluster Of Differentiation 40]
other names :
[CD40 ligand; CD40 ligand; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154]
products gene name :
[CD40L]
other gene names :
[CD40LG; CD40LG; CD40L; CD40L; TNFSF5; CD40-L]
reactivity :
Rhesus monkey
specificity :
The antibody is a Rabbit polyclonal antibody raised against CD40L. It has been selected for its ability to recognize CD40L in immunohistochemical staining and western blotting.
purity :
Antigen-specific Affinity Chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP.
app notes :
Western blotting: 0.5-2µg/mL. Immunocytochemistry in formalin fixed cells: 5-20µg/mL. Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL. Immunohistochemistry in paraffin section: 5-20µg/mL. Optimal working dilutions must be determined by end user
image1 heading :
Western Blot (WB)
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CD40L (Asn180~Leu261) expressed in E.coli.
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2038135
ncbi acc num :
NP_001028011.1
ncbi gb acc num :
NM_001032839.1
ncbi mol weight :
29,366 Da
ncbi pathways :
Allograft Rejection Pathway (86789); Allograft Rejection Pathway (535); Asthma Pathway (86786); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (86787); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (86739); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine-cytokine Receptor Interaction Pathway (86721); Cytokine-cytokine Receptor Interaction Pathway (460)
uniprot summary :
Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.