catalog number :
MBS2028566
products full name :
Polyclonal Antibody to Protein Kinase Inhibitor Beta (PKIb)
products short name :
[Protein Kinase Inhibitor Beta]
other names :
[cAMP-dependent protein kinase inhibitor beta isoform 1; cAMP-dependent protein kinase inhibitor beta; cAMP-dependent protein kinase inhibitor beta; cAMP-dependent protein kinase inhibitor beta]
products gene name :
[PKIb]
other gene names :
[PKIB; PKIB; PRKACN2; PRKACN2; PKI-beta]
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
tested application :
Western Blot(WB), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin(IHC-P), Immunohistochemistry-Frozen (IHC-F), ELISA (EIA).
app notes :
Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200.
Optimal working dilutions must be determined by end user
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Immunohistochemistry (IHC)
image4 heading :
Immunohistochemistry (IHC)
image4 description :
DAB staining on IHC-P; Samples: Human Kidney Tissue
image5 heading :
Immunohistochemistry (IHC)
image5 description :
DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.
other info1 :
Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant PKIb (Met8~Glu76 (Accession # Q9C010)) expressed in E.coli
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2041336
ncbi acc num :
NP_001257322.1
ncbi gb acc num :
NM_001270393.1
ncbi pathways :
Calcium Regulation In The Cardiac Cell Pathway (198906); Myometrial Relaxation And Contraction Pathways (198759)
ncbi summary :
This gene encodes a member of the cAMP-dependent protein kinase inhibitor family. The encoded protein may play a role in the protein kinase A (PKA) pathway by interacting with the catalytic subunit of PKA, and overexpression of this gene may play a role in prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
uniprot summary :
PKIB: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. Belongs to the PKI family. Protein type: Inhibitor; Protein kinase, regulatory subunit. Chromosomal Location of Human Ortholog: 6q22.31. Cellular Component: cytoplasm; nucleus. Molecular Function: cAMP-dependent protein kinase inhibitor activity. Biological Process: positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase