catalog number :
MBS2028490
products full name :
Polyclonal Antibody to Complement Component 3 (C3)
products short name :
[Complement Component 3]
other names :
[complement C3; Complement C3; complement C3; Complement C3 beta chainC3-beta-c; C3bc]
products gene name :
[C3]
other gene names :
[C3; C3; C3d; C3bc; ASP]
reactivity :
Porcine (Pig)
specificity :
The antibody is a Rabbit polyclonal antibody raised against C3. It has been selected for its ability to recognize C3 in immunohistochemical staining and western blotting.
purity :
Antigen-specific Affinity Chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP
app notes :
Western blotting: 0.5-2µg/mL. Immunohistochemistry: 5-20µg/mL. Immunocytochemistry: 5-20µg/mL. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Immunohistochemistry (IHC)
image4 heading :
Immunohistochemistry (IHC)
image4 description :
DAB staining on IHC-P; Samples: Porcine Liver Tissue)
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant C3 (Asp23~Thr300) expressed in E.coli
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2048845
ncbi acc num :
NP_999174.1
ncbi gb acc num :
NM_214009.1
ncbi mol weight :
186,807 Da
ncbi pathways :
Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Complement And Coagulation Cascades Pathway (84485); Complement And Coagulation Cascades Pathway (484); Herpes Simplex Infection Pathway (377870); Herpes Simplex Infection Pathway (377865); Legionellosis Pathway (469196); Legionellosis Pathway (469186); Leishmaniasis Pathway (144174); Leishmaniasis Pathway (144028)
uniprot summary :
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates ().