Polyclonal Antibody to Cluster Of Differentiation 8a (CD8a) | MyBioSource MBS2028386 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Cluster Of Differentiation 8a (CD8a)

catalog :

MBS2028386

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2028386

products type :

Antibody

products full name :

Polyclonal Antibody to Cluster Of Differentiation 8a (CD8a)

products short name :

Cluster Of Differentiation 8a

other names :

CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2; CD_antigen: CD8a

products gene name :

CD8a

other gene names :

CD8A; CD8A; CD8; MAL; p32; Leu2; MAL

uniprot entry name :

CD8A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

235

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-AKPQ APELRIFPKK MDAELGQKVD LVCEVLGSVS QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK LFSAMRDTNN KYVLTLNKFS KENEGYYFCS VISNSVMYFS SVVPVLQKVN STTTKPVLRT PSPVHPTGTS QPQRPEDCRP RGSVKGTGLD FACDIYIWAP LAGICVALLL

specificity :

The antibody is a rabbit polyclonal antibody raised against CD8a. It has been selected for its ability to recognize CD8a in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-5000. Optimal working dilutions must be determined by end user.

other info1 :

Fragment: CD8a (Ala27~Val247). Organism Species: Mus musculus (Mouse)

other info2 :

Immunogen: CD8a, Mouse. Immunogen Information: Recombinant CD8a (Ala27~Val247) with two N-terminal Tags, His-tag and GST-tag expressed in E.coli. Accession No.: RPB099Mu01

ncbi gi num :

19344022

ncbi acc num :

AAH25715.1

ncbi mol weight :

30,214 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (1269170)

ncbi summary :

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; MHC class I protein binding; coreceptor activity; protein kinase binding. Biological Process: antigen processing and presentation; regulation of immune response; T cell activation; T cell mediated immunity; positive regulation of calcium-mediated signaling; immune response; cytotoxic T cell differentiation; defense response to virus; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Cd8 Deficiency, Familial

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

230

size3 :

0.1 mg

price3 :

310

more info or order :

MyBioSource product webpage