catalog number :
MBS2028168
products full name :
Polyclonal Antibody to Ficolin 2 (FCN2)
products short name :
[Ficolin 2]
other names :
[ficolin-2; Ficolin-2; ficolin-2; ficolin B; Collagen/fibrinogen domain-containing protein 2; Ficolin-B; Ficolin-beta; L-ficolin]
products gene name :
[FCN2]
other gene names :
[Fcnb; Fcn2; Fcn2; Fcnb]
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
tested application :
Western blotting (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
app notes :
Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant FCN2 (Gln97~Ser254) expressed in E.coli
ncbi acc num :
NP_034320.1
ncbi gb acc num :
NM_010190.1
ncbi pathways :
Complement Cascade Pathway (1001237); Creation Of C4 And C2 Activators Pathway (1001239); Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway (1001241); Immune System Pathway (971216); Initial Triggering Of Complement Pathway (1001238); Innate Immune System Pathway (971212); Lectin Pathway Of Complement Activation (1001240)
uniprot summary :
FCN2: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. Belongs to the ficolin lectin family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 2 2 A3. Cellular Component: collagen; extracellular region; extrinsic to external side of plasma membrane. Molecular Function: carbohydrate binding; G-protein coupled receptor binding; metal ion binding; sialic acid binding; signaling pattern recognition receptor activity. Biological Process: cell surface pattern recognition receptor signaling pathway; complement activation, lectin pathway; G-protein coupled receptor signaling pathway; immune system process; innate immune response; negative regulation of virion penetration into host cell; recognition of apoptotic cell