Polyclonal Antibody to Serine/Arginine Rich Splicing Factor 2 (SRSF2) | MyBioSource MBS2028053 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Serine/Arginine Rich Splicing Factor 2 (SRSF2)

catalog :

MBS2028053

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant protein.

image 2 :

DABstainingonIHC-P.Samples:HumanTissue)

product information

catalog number :

MBS2028053

products type :

Antibody

products full name :

Polyclonal Antibody to Serine/Arginine Rich Splicing Factor 2 (SRSF2)

products short name :

[Serine/Arginine Rich Splicing Factor 2]

other names :

[serine/arginine-rich splicing factor 2; Serine/arginine-rich splicing factor 2; serine/arginine-rich splicing factor 2; serine and arginine rich splicing factor 2; Protein PR264; Splicing component, 35 kDa; Splicing factor SC35; SC-35; Splicing factor, arginine/serine-rich 2]

products gene name :

[SRSF2]

other gene names :

[SRSF2; SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b; SFRS2; SC-35]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

0.42 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2)Ug/mL;1:200-800. Immunohistochemistry: 5-20 Ug/mL;1:20-80. Immunocytochemistry: 5-20 ug/mL;1:20-80. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant SRSF2 (Thr14~Ser221) expressed in E.coli

ncbi gi num :

306482646

ncbi acc num :

NP_001182356.1

ncbi gb acc num :

NM_001195427.1

uniprot acc num :

Q01130

ncbi pathways :

Cleavage Of Growing Transcript In The Termination Region Pathway (1269686); Gene Expression Pathway (1269649); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688); RNA Polymerase II Transcription Pathway (1269675); RNA Polymerase II Transcription Termination Pathway (1269685); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Transport Of Mature Transcript To Cytoplasm Pathway (1269693)

ncbi summary :

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010]

uniprot summary :

SFRS2: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Belongs to the splicing factor SR family. Protein type: RNA processing; RNA splicing; Spliceosome. Chromosomal Location of Human Ortholog: 17q25.1. Cellular Component: cytosol; extracellular exosome; nuclear speck; nucleoplasm; nucleus; PML body; spliceosomal complex. Molecular Function: protein binding; protein kinase C binding; RNA binding; transcription corepressor activity. Biological Process: mitotic cell cycle; mRNA 3'-end processing; mRNA export from nucleus; mRNA processing; mRNA splicing, via spliceosome; regulation of alternative mRNA splicing, via spliceosome; response to vitamin E; RNA export from nucleus; RNA splicing; termination of RNA polymerase II transcription

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

340

size6 :

1 mg

price6 :

800

more info or order :

MyBioSource product webpage