product summary
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company name :
MyBioSource
product type :
antibody
product name :
Polyclonal Antibody to Chordin (CHRD)
catalog :
MBS2028039
quantity :
0.01 mg
price :
110 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS2028039 image 1
Western Blot: Sample: Mouse Lung Tissue.
image 2 :
MyBioSource MBS2028039 image 2
Western Blot: Sample: Recombinant CHRD, Mouse.
image 3 :
MyBioSource MBS2028039 image 3
DAB staining on IHC-P; Samples: Mouse Heart Tissue.
product information
catalog number :
MBS2028039
products type :
Antibody
products full name :
Polyclonal Antibody to Chordin (CHRD)
products short name :
[Chordin]
other names :
[chordin isoform 2; Chordin; chordin; chordin]
products gene name :
[CHRD]
other gene names :
[Chrd; Chrd; Chd]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Mus musculus (Mouse)
sequence length :
81
sequence :
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SASFILLGN GSLIYQVQVV GTGSEVVAMT LETKPQRKNQ RTVLCHMAGL QPGGHMAVGM CSGLGARGAH MLLQNELFLN VGTKDFPDGE LRGHVTALCY SGHSARYDRL PVPLAGALVL PPVRSQAAGH AWLSLDTHCH LHYEVLLAGL GGSEQGTVTA HLLGPPGMPG PQRLLKGFYG SEAQGVVKDL
specificity :
The antibody is a rabbit polyclonal antibody raised against CHRD. It has beenselected for its ability to recognize CHRD in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
concentration :
200ug/ml
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
app notes :
Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-5000. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Immunohistochemistry (IHC)
image4 heading :
Immunohistochemistry (IHC)
image4 description :
DAB staining on IHC-P; Samples: Mouse Intestine Tissue
image5 heading :
Immunohistochemistry (IHC)
image5 description :
DAB staining on IHC-P; Samples: Mouse Kidney Tissue
other info1 :
Immunogen: CHRD, Mouse. Immunogen: Recombinant CHRD (Ser422~Gly635) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPC126Mu01
other info2 :
Conjugate: No Conjugate. Immunogen: Recombinant CHRD (Ser422~Gly635) expressed in E Coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2063379
ncbi gi num :
487442580
ncbi acc num :
NP_001264970.1
ncbi gb acc num :
NM_001278041.1
uniprot acc num :
Q9Z0E2
ncbi mol weight :
101,513 Da
ncbi pathways :
TGF-beta Signaling Pathway (83261); TGF-beta Signaling Pathway (475)
uniprot summary :
CHRD: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Belongs to the chordin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16 B1 16 12.51 cM. Cellular Component: extracellular region; extracellular space. Molecular Function: heparin binding; protein binding; syndecan binding. Biological Process: BMP signaling pathway in spinal cord dorsal-ventral patterning; central nervous system development; dorsal/ventral pattern formation; forebrain development; gastrulation with mouth forming second; mesoderm formation; multicellular organism development; negative regulation of BMP signaling pathway; negative regulation of cell migration; negative regulation of osteoblast differentiation; osteoblast differentiation; pattern specification process; positive regulation of cell adhesion; positive regulation of mesenchymal cell proliferation; skeletal system development
size1 :
0.01 mg
price1 :
110 USD
size2 :
0.02 mg
price2 :
130
size3 :
0.05 mg
price3 :
185
size4 :
0.1 mg
price4 :
230
size5 :
0.2 mg
price5 :
345
size6 :
1 mg
price6 :
825
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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