Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1) | MyBioSource MBS2027881 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1)

catalog :

MBS2027881

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. Western Blot; Sample: Recombinant CYP27B1, Mouse.

image 2 :

DAB staining on fromalin fixed paraffin-embedded Kidney tissue)

product information

catalog number :

MBS2027881

products type :

Antibody

products full name :

Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1)

products short name :

[Cytochrome P450 27B1]

other names :

[25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; cytochrome P450, family 27, subfamily b, polypeptide 1; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1]

products gene name :

[CYP27B1]

other gene names :

[Cyp27b1; Cyp27b1; Vdr; Cp2b; Cyp1; Pddr; Vdd1; Vddr; Cyp40; VddrI; Cyp27b; P450c1; Cyp27b; Cyp40; VD3 1A hydroxylase]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

0.71 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:350-1400. Immunohistochemistry: 5-20 ug/mL;1:35-140. Immunocytochemistry: 5-20 ug/mL;1:35-140. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CYP27B1 (Asp258~Phe504 (Accession # O35084)) expressed in E.coli.

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2071775

ncbi gi num :

227497562

ncbi acc num :

NP_034139.2

ncbi gb acc num :

NM_010009.2

uniprot acc num :

O35084

ncbi pathways :

1,25-dihydroxyvitamin D3 Biosynthesis Pathway (542888); Biological Oxidations Pathway (1367854); Cholecalciferol Biosynthesis Pathway (421791); Cholecalciferol Biosynthesis Pathway (468294); Cytochrome P450 - Arranged By Substrate Type Pathway (1367856); Metabolic Pathways (132962); Metabolism Pathway (1367614); Metabolism Of Fat-soluble Vitamins Pathway (1367813); Metabolism Of Vitamins And Cofactors Pathway (1367799); Metapathway Biotransformation (198377)

uniprot summary :

CYP27B1: Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. Defects in CYP27B1 are the cause of rickets vitamin D- dependent type 1A (VDDR1A); also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25- dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. Belongs to the cytochrome P450 family. Protein type: Cell cycle regulation; EC 1.14.13.13; Lipid Metabolism - steroid biosynthesis; Mitochondrial; Oxidoreductase. Chromosomal Location of Human Ortholog: 10 10 D3. Cellular Component: cytoplasm; membrane; mitochondrion. Molecular Function: calcidiol 1-monooxygenase activity; heme binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. Biological Process: aging; calcium ion homeostasis; calcium ion transport; negative regulation of cell growth; negative regulation of cell proliferation; positive regulation of keratinocyte differentiation; regulation of bone mineralization; response to cAMP; response to copper ion; response to drug; response to insulin stimulus; response to lipopolysaccharide; response to vitamin D; vitamin D catabolic process; vitamin D metabolic process

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

185

size4 :

0.1 mg

price4 :

230

size5 :

0.2 mg

price5 :

345

size6 :

1 mg

price6 :

825

more info or order :

MyBioSource product webpage