Polyclonal Antibody to Early B-Cell Factor 1 (EBF1) | MyBioSource MBS2027769 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Early B-Cell Factor 1 (EBF1)

catalog :

MBS2027769

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Mouse Sp2/0 cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human EBF1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

image 2 :

Western Blot: Sample: Recombinant protein.

image 3 :

DAB staining on fromalin fixed paraffin-embedded Heart tissue)

product information

catalog number :

MBS2027769

products type :

Antibody

products full name :

Polyclonal Antibody to Early B-Cell Factor 1 (EBF1)

products short name :

[Early B-Cell Factor 1]

other names :

[transcription factor COE1 isoform 2; Transcription factor COE1; transcription factor COE1; early B cell factor 1; Early B-cell factor]

products gene name :

[EBF1]

other gene names :

[EBF1; EBF1; EBF; COE1; OLF1; O/E-1; COE1; EBF; O/E-1; OE-1]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence :

Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- DP VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ LPALANTSVH

specificity :

The antibody is a rabbit polyclonal antibody raised against EBF1. It has beenselected for its ability to recognize EBF1 in immunohistochemical staining andwestern blotting.

purity :

Antigen-specific Affinity Chromatography.

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), IP, IF, FCM.

app notes :

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Knockout Validation

image4 description :

Knockout Validation: Lane 1: Wild-type Raji cell lysate; Lane 2: EBF1 knockout Raji cell lysate; Predicted MW: 64kDa Observed MW: 60kDa Primary Ab: 3ug/ml Rabbit Anti-Human EBF1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

other info1 :

Fragment: EBF1 (Asp179~Ser451). Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation

other info2 :

Conjugate: No Conjugate. Immunogen: Recombinant Early B-Cell Factor 1 (EBF1). Traits: Liquid. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2055011

products categories :

Knock-Out Validation Antibody

ncbi gi num :

31415878

ncbi acc num :

NP_076870.1

ncbi gb acc num :

NM_024007.4

uniprot acc num :

Q9UH73

ncbi mol weight :

61,059 Da

ncbi pathways :

Adipogenesis Pathway (198832); Developmental Biology Pathway (477129); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (205243)

uniprot summary :

EBF1: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription regulation. Chromosomal Location of Human Ortholog: 5q33.3. Cellular Component: nucleus. Molecular Function: DNA binding; metal ion binding; protein binding. Biological Process: multicellular organism development; regulation of transcription, DNA-templated; transcription, DNA-dependent

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage