catalog number :
MBS2027557
products full name :
Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)
products short name :
[Chymase 1]
other names :
[chymase; Chymase; chymase; chymase 1; Alpha-chymase; Mast cell protease 3; rMCP-3; Mast cell protease 5; rMCP-5; Mast cell protease III; rMCP-III]
products gene name :
[CMA1]
other gene names :
[Cma1; Cma1; MCP3P; Mcpt5; Mcpt3; rMCP-3; rMCP-5; rMCP-III]
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
concentration :
0.91mg/ml
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP.
app notes :
Western blotting: 0.5-2(Ug/mL;1:450-1800. Immunohistochemistry: 5-20(Ug/mL;1:45-180. Immunocytochemistry: 5-20(Ug/mL;1:45-180.
Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Organism Species: Rattus norvegicus (Rat). Source: Polyclonal antibody preparation. Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography. Traits: Liquid. Immunogen: Recombinant CMA1 (Ile22~Asn247 (Accession # P50339)) expressed in E.coli
other info2 :
Conjugated Antibody: The APC-Cy7 conjugated antibody version of this item is also available as catalog #MBS2078264
ncbi acc num :
NP_037224.1
ncbi gb acc num :
NM_013092.1
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1376640); Degradation Of The Extracellular Matrix Pathway (1376639); Extracellular Matrix Organization Pathway (1376626); Metabolism Of Angiotensinogen To Angiotensins Pathway (1377536); Metabolism Of Proteins Pathway (1377481); Peptide Hormone Metabolism Pathway (1377533); Renin - Angiotensin System Pathway (219775); Renin-angiotensin System Pathway (83467); Renin-angiotensin System Pathway (486); Signal Transduction Pathway (1376816)
ncbi summary :
connective tissue mast cell protein with proteolytic activity; may facilitate apoptosis [RGD, Feb 2006]
uniprot summary :
CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: EC 3.4.21.39; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 15p13. Cellular Component: extracellular matrix; extracellular region; intracellular; secretory granule. Molecular Function: endopeptidase activity; peptidase activity; peptide binding; serine-type endopeptidase activity; serine-type peptidase activity. Biological Process: interleukin-1 beta biosynthetic process; midbrain development; peptide metabolic process; positive regulation of angiogenesis; proteolysis