catalog number :
MBS2027456
products full name :
Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)
products short name :
[Cluster Of Differentiation 14]
other names :
[Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein]
products gene name :
[CD14]
other gene names :
[CD14]
reactivity :
Porcine (Pig)
specificity :
The antibody is a Rabbit polyclonal antibody raised against CD14. It has been selected for its ability to recognize CD14 in immunohistochemical staining and western blotting.
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP
app notes :
Western blotting: 0.5-2ug/mL. Immunohistochemistry: 5-20ug/mL. Immunocytochemistry: 5-20ug/mL. Optimal working dilutions must be determined by end user
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Western Blot (WB)
image4 heading :
Immunohistochemistry (IHC)
image4 description :
DAB staining on IHC-P; Samples: Porcine Liver Tissue)
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CD14 (Thr21~Ala373) expressed in E.coli
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2046015
ncbi mol weight :
39,770 Da
uniprot summary :
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL-.