catalog number :
MBS2027117
products full name :
Polyclonal Antibody to Haptoglobin (Hpt)
products short name :
[Haptoglobin]
other names :
[haptoglobin; Haptoglobin; haptoglobin; Zonulin]
products gene name :
[Hpt]
other gene names :
[HP; HP]
reactivity :
Bovine (Cattle)
specificity :
The antibody is a Rabbit polyclonal antibody raised against Hpt. It has been selected for its ability to recognize Hpt in immunohistochemical staining and western blotting.
purity :
Antigen-specific Affinity Chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP
app notes :
Western blotting: 0.5-5µg/mL. Immunohistochemistry: 5-20µg/mL. Immunocytochemistry: 5-20µg/mL. Optimal working dilutions must be determined by end user
image1 heading :
Western Blot (WB)
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant Hpt (Gly159~Leu329) expressed in E.coli
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2047943
ncbi acc num :
NP_001035560.1
ncbi gb acc num :
NM_001040470.2
ncbi mol weight :
44,859 Da
ncbi pathways :
Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1376115); Immune System Pathway (1376247); Innate Immune System Pathway (1376277); Neutrophil Degranulation Pathway (1458149); Scavenging Of Heme From Plasma Pathway (1376116); Vesicle-mediated Transport Pathway (1376091)
uniprot summary :
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway ().