Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit B (SDHB) | MyBioSource MBS2027081 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit B (SDHB)

catalog :

MBS2027081

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse SDHB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

image 2 :

Western Blot: Sample: Recombinant protein.

image 3 :

DAB staining on fromalin fixed paraffin- embedded brain tissue)

product information

catalog number :

MBS2027081

products type :

Antibody

products full name :

Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit B (SDHB)

products short name :

[Succinate Dehydrogenase Complex Subunit B]

other names :

[succinate dehydrogenase; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex, subunit B, iron sulfur (Ip); Iron-sulfur subunit of complex II; Ip]

products gene name :

[SDHB]

other gene names :

[Sdhb; Sdhb; 0710008N11Rik; Ip]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

0.79 mg/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2 ug/mL;1:400-1600. Immunohistochemistry: 5-20 ug/mL;1:40-160. Immunocytochemistry: 5-20 ug/mL;1:40-160. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant SDHB (Glu97~Ala282) expressed in E.coli

ncbi gi num :

34328286

ncbi acc num :

NP_075863.2

ncbi gb acc num :

NM_023374.4

uniprot acc num :

Q9CQA3

ncbi pathways :

Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Carbon Metabolism Pathway (815838); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (83127); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855821); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (421748); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204)

uniprot summary :

SDHB: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC). A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. Defects in SDHB are the cause of paragangliomas type 4 (PGL4). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Defects in SDHB are a cause of Cowden-like syndrome (CWDLS). Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.3.5.1; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase. Chromosomal Location of Human Ortholog: 4 4 D3. Cellular Component: membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex II; mitochondrion; nucleoplasm; plasma membrane. Molecular Function: 2 iron, 2 sulfur cluster binding; 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; electron transfer activity; iron-sulfur cluster binding; metal ion binding; oxidoreductase activity; succinate dehydrogenase (ubiquinone) activity. Biological Process: aerobic respiration; succinate metabolic process; transport; tricarboxylic acid cycle

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

185

size4 :

0.1 mg

price4 :

230

size5 :

0.2 mg

price5 :

345

size6 :

1 mg

price6 :

825

more info or order :

MyBioSource product webpage