Polyclonal Antibody to Agrin (AGRN) | MyBioSource MBS2026614 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Agrin (AGRN)

catalog :

MBS2026614

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant protein.

image 2 :

DABstainingonIHC-P.Samples:HumanTissue)

product information

catalog number :

MBS2026614

products type :

Antibody

products full name :

Polyclonal Antibody to Agrin (AGRN)

products short name :

[Agrin]

other names :

[agrin isoform 2; Agrin; agrin; agrin]

products gene name :

[AGRN]

other gene names :

[AGRN; AGRN; CMS8; AGRIN; CMSPPD; AGRIN; C90; C22]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography!!Traits Liquid!!

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2 Ug/mL;1:500-2000!!Immunohistochemistry: 5-20 Ug/mL;1:50-200!!Immunocytochemistry: 5-20 Ug/mL;1:50-200. Optimal working dilutions must be determined by end user

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Immunogen: Recombinant AGRN (Leu1856~Pro2045) expressed in E.coli

ncbi gi num :

54873613

ncbi acc num :

NP_940978.2

ncbi gb acc num :

NM_198576.3

uniprot acc num :

O00468

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1269981); Axon Guidance Pathway (1270303); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway (1309217); Defective B3GAT3 Causes JDSSDHD Pathway (1269015); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1269014); Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway (1269021); Defective EXT2 Causes Exostoses 2 Pathway (1269022); Developmental Biology Pathway (1270302); Disease Pathway (1268854)

ncbi summary :

This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

uniprot summary :

agrin: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function. Protein type: Cell adhesion; Cell development/differentiation; Extracellular matrix; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.33. Cellular Component: basal lamina; cell junction; cytosol; extracellular matrix; extracellular region; Golgi lumen; integral component of membrane; lysosomal lumen; plasma membrane; synapse. Molecular Function: calcium ion binding; heparan sulfate proteoglycan binding; laminin binding; protein binding; sialic acid binding; structural constituent of cytoskeleton. Biological Process: clustering of voltage-gated sodium channels; cytoskeleton organization; extracellular matrix organization; G-protein coupled acetylcholine receptor signaling pathway; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; receptor clustering; retinoid metabolic process; signal transduction; synapse organization. Disease: Myasthenic Syndrome, Congenital, With Pre- And Postsynaptic Defects

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage