Polyclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A) | MyBioSource MBS2026219 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A)

catalog :

MBS2026219

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Lane1: Human Lung Tissue; Lane2: Human Hela Cells.

image 2 :

Western Blot: Sample: Recombinant PLA2G2A, Human.

image 3 :

DAB staining on IHC-P. Samples: Human Tissue)

product information

catalog number :

MBS2026219

products type :

Antibody

products full name :

Polyclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A)

products short name :

[Phospholipase A2]

other names :

[phospholipase A2, membrane associated; Phospholipase A2, membrane associated; phospholipase A2, membrane associated; phospholipase A2 group IIA; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A]

products gene name :

[PLA2G2A]

other gene names :

[PLA2G2A; PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; PLA2B; PLA2L; RASF-A; NPS-PLA2]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

144

sequence :

Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-NLVNFHRMIK LTTGKEAALS YGFYGCHCGV GGRGSPKDAT DRCCVTHDCC YKRLEKRGCG TKFLSYKFSN

specificity :

The antibody is a rabbit polyclonal antibody raised against PLA2G2A. It has beenselected for its ability to recognize PLA2G2A in immunohistochemical staining andwestern blotting.

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

200ug/ml

storage stability :

Storage: . Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test:. The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2ug/mL. Immunohistochemistry: 5-20ug/mL. Immunocytochemistry: 5-20ug/mL. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Source: Polyclonal antibody preparation. Traits: Liquid

other info2 :

Immunogen: Recombinant PLA2G2A (Asn21~Cys144) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2073085

ncbi gi num :

4505849

ncbi acc num :

NP_000291.1

ncbi gb acc num :

NM_000300.3

uniprot acc num :

P14555

ncbi mol weight :

16,083 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

ncbi summary :

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

uniprot summary :

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:2925633). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins (PubMed:18635536, PubMed:25398877). Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 (PubMed:18635536, PubMed:25398877). Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:25398877). Induces cell proliferation in an integrin-dependent manner (PubMed:18635536).

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

340

size6 :

1 mg

price6 :

800

more info or order :

MyBioSource product webpage