Polyclonal Antibody to Complement Factor I (CFI) | MyBioSource MBS2025927 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Complement Factor I (CFI)

catalog :

MBS2025927

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot; Sample: Mouse Small intestine lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CFI Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)

image 2 :

Western Blot: Sample: Recombinant CFI, Mouse.

image 3 :

Western Blot; Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CFI Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)

product information

catalog number :

MBS2025927

products type :

Antibody

products full name :

Polyclonal Antibody to Complement Factor I (CFI)

products short name :

[Complement Factor I]

other names :

[complement factor I isoform 1 preproprotein; Complement factor I; complement factor I; complement component factor i; C3B/C4B inactivator]

products gene name :

[CFI]

other gene names :

[Cfi; Cfi; If]

clonality :

Polyclonal

host :

Rabbit

sequence length :

604

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CFI (Arg358~Val603) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2061445

ncbi gi num :

110347406

ncbi acc num :

NP_031712.2

ncbi gb acc num :

NM_007686.3

uniprot acc num :

Q61129

ncbi pathways :

Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (819634); Immune System Pathway (819567); Innate Immune System Pathway (819596); Regulation Of Complement Cascade Pathway (819643); Staphylococcus Aureus Infection Pathway (172855); Staphylococcus Aureus Infection Pathway (171867)

ncbi summary :

This gene encodes a serine protease that plays an important role in the classical and alternative complement pathways where it cleaves C4b and C3b components of C3 and C5 convertases. The encoded preproprotein undergoes proteolytic processing to generate an active, disulfide-linked heterodimeric enzyme comprised of heavy and light chains. [provided by RefSeq, Jul 2016]

uniprot summary :

CFI: Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively. Defects in CFI are a cause of susceptibility to hemolytic uremic syndrome atypical type 3 (AHUS3). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Defects in CFI are the cause of complement factor I deficiency (CFI deficiency). CFI deficiency is an autosomal recessive condition associated with a propensity to pyogenic infections. Belongs to the peptidase S1 family. Protein type: EC 3.4.21.45; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3 G3 3 59.21 cM. Cellular Component: extracellular region; extracellular space; membrane; nucleus. Molecular Function: hydrolase activity; metal ion binding; peptidase activity; scavenger receptor activity; serine-type endopeptidase activity; serine-type peptidase activity. Biological Process: complement activation, classical pathway; immune system process; innate immune response; proteolysis

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

330

size6 :

1 mg

price6 :

785

more info or order :

MyBioSource product webpage