antibody

Monoclonal Antibody to Neuropilin 1 (NRP1)

MBS2025850

0.01 mg

100 USD

monoclonal

mouse

nonconjugated

[4#]

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Antibody

Monoclonal Antibody to Neuropilin 1 (NRP1)

[Neuropilin 1]

[neuropilin-1 isoform b; Neuropilin-1; neuropilin-1; neuropilin 1; Vascular endothelial cell growth factor 165 receptor; CD_antigen: CD304]

[NRP1]

[NRP1; NRP1; NP1; NRP; BDCA4; CD304; VEGF165R; NRP; VEGF165R]

Monoclonal

IgG2b Kappa

[4#]

Mouse

Human

The antibody is a mouse monoclonal antibody raised against NRP1. It has beenselected for its ability to recognize NRP1 in immunohistochemical staining andwestern blotting.

Protein A/G Affinity Chromatography.

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

500ug/ml

Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.

Western blotting: 0.5-5ug/ml. Immunocytochemistry in formalin fixed cells: 5-30ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml. Immunohistochemistry in paraffin section: 5-30ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

Western Blot (WB)

Source: Monoclonal antibody preparation. Traits: Liquid

Immunogen: Recombinant NRP1 (Tyr646~Asp814) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2046121

66912178

NP_001019799.1

NM_001024628.2

O14786

68,376 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CHL1 Interactions Pathway (161007); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); L1CAM Interactions Pathway (161003); Neurophilin Interactions With VEGF And VEGFR Pathway (106509)

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 10p11.22. Cellular Component: axon; cell soma; cell surface; cytoplasmic vesicle; cytosol; early endosome; extracellular space; focal adhesion; growth cone; integral component of membrane; neurofilament; neuron projection; plasma membrane; receptor complex. Molecular Function: coreceptor activity; cytokine binding; growth factor binding; GTPase activator activity; heparin binding; metal ion binding; protein binding; semaphorin receptor activity; vascular endothelial growth factor binding; vascular endothelial growth factor-activated receptor activity. Biological Process: actin cytoskeleton reorganization; angiogenesis; artery morphogenesis; axon extension involved in axon guidance; axon guidance; axonal fasciculation; branching involved in blood vessel morphogenesis; branchiomotor neuron axon guidance; cell migration during sprouting angiogenesis; cell-cell signaling; commissural neuron axon guidance; dendrite arborization; dendrite development; dorsal root ganglion morphogenesis; endothelial cell migration; facial nerve structural organization; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; hepatocyte growth factor receptor signaling pathway; integrin-mediated signaling pathway; negative regulation of axon extension involved in axon guidance; negative regulation of extrinsic apoptotic signaling pathway; negative regulation of neuron apoptosis; nerve development; neural crest cell migration involved in autonomic nervous system development; neuron migration; neuropilin signaling pathway; organ morphogenesis; otic placode development; outflow tract septum morphogenesis; platelet-derived growth factor receptor signaling pathway; positive chemotaxis; positive regulation of actin cytoskeleton reorganization; positive regulation of axon extension involved in axon guidance; positive regulation of cell migration involved in sprouting angiogenesis; positive regulation of endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of filopodium formation; positive regulation of focal adhesion assembly; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of retinal ganglion cell axon guidance; positive regulation of smooth muscle cell migration; positive regulation of stress fiber formation; positive regulation of substrate adhesion-dependent cell spreading; regulation of Cdc42 protein signal transduction; regulation of retinal ganglion cell axon guidance; response to wounding; retinal ganglion cell axon guidance; semaphorin-plexin signaling pathway; semaphorin-plexin signaling pathway involved in axon guidance; semaphorin-plexin signaling pathway involved in neuron projection guidance; signal transduction; sprouting angiogenesis; substrate-dependent cell migration, cell extension; toxin transport; trigeminal nerve structural organization; vascular endothelial growth factor receptor signaling pathway; VEGF-activated neuropilin signaling pathway; ventral trunk neural crest cell migration; vestibulocochlear nerve structural organization

0.01 mg

100 USD

0.02 mg

110

0.05 mg

140

0.1 mg

170

0.2 mg

220

1 mg

495